LOCUS       sequence-177076-        8111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu 
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE
  TITLE     Optogenetic control with a photocleavable protein, PhoCl.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222.
  PUBMED    28288123
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222."
COMMENT     Sequence Label: pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
FEATURES             Location/Qualifiers
     source          1..8111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..36
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     98..117
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             165..1100
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1116..1841
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             2835..3560
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             3573..4508
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3621..4514
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(4602..4621)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4667..4722
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4721..4740)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5083..5099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5083..5099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5096..5118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5107..5123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5131..5137,5138..5155,5156..5161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5176..5197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5256..5451
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5302..5437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5457..5591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5494..5513)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5548..5567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5659..5676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5830..6418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5910..5929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6589..7380,7381..7449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASWQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYMTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7212..7231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7450..7554)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7585..7964
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
       61 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
      121 tctcatcatt ttggcaaaga attcccgggt gagccgccac catggctgga gacatgagag
      181 ctgccaacct ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct
      241 tgtccctgac ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct
      301 attccgagta tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca
      361 acctggcaga cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg
      421 tggatttgac cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga
      481 tgattggtct cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact
      541 tgctcttgga caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc
      601 tgctggctac atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc
      661 tcaaatctat tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt
      721 ctctggaaga gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc
      781 acctgatggc caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc
      841 tcctcatcct ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca
      901 tgaagtgcaa gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc
      961 gcctacatgc gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact
     1021 tggccactgc gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg
     1081 cagagggttt ccctgccaca gctgtcgacg gtaccgtgat ccctgactac ttcaagcaga
     1141 gcttccccga gggctacagc tgggagcgca gcatgaccta cgaggacggc ggcatctgca
     1201 tcgccaccaa cgacatcaca atggaggggg acagcttcat caacaagatc cacttcaagg
     1261 gcacgaactt cccccccaac ggccccgtga tgcagaagag gaccgtgggc tgggaggcca
     1321 gcaccgagaa gatgtacgag cgcgacggcg tgctgaaggg cgacgtgaag atgaagctgc
     1381 tgctgaaggg cggcggccac tatcgctgcg actaccgcac cacctacaag gtcaagcaga
     1441 agcccgtaaa gctgcccgac taccacttcg tggaccaccg catcgagatc ctgagccacg
     1501 acaaggacta caacaaggtg aagctgtacg agcacgccgt ggcccgcaac tccaccgaca
     1561 gcatggacga gctgtacaag ggtggcagcg gtggcatggt gagcaagggc gaggagacca
     1621 ttacaagcgt gatcaagcct gacatgaaga acaagctgcg catggagggc aacgtgaacg
     1681 gccacgcctt cgtgatcgag ggcgagggca gcggcaagcc cttcgagggc atccagacga
     1741 ttgatttgga ggtgaaggag ggcgccccgc tgcccttcgc ctacgacatc ctgaccaccg
     1801 ccttccacta cggcaaccgc gtgttcacca agtacccacg ggctagcgat gcaacgagtg
     1861 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
     1921 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
     1981 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
     2041 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
     2101 ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
     2161 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
     2221 atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc
     2281 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
     2341 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
     2401 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
     2461 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
     2521 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
     2581 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
     2641 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
     2701 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
     2761 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
     2821 atggcgattc cggagtgatc cctgactact tcaagcagag cttccccgag ggctacagct
     2881 gggagcgcag catgacctac gaggacggcg gcatctgcat cgccaccaac gacatcacaa
     2941 tggaggggga cagcttcatc aacaagatcc acttcaaggg cacgaacttc ccccccaacg
     3001 gccccgtgat gcagaagagg accgtgggct gggaggccag caccgagaag atgtacgagc
     3061 gcgacggcgt gctgaagggc gacgtgaaga tgaagctgct gctgaagggc ggcggccact
     3121 atcgctgcga ctaccgcacc acctacaagg tcaagcagaa gcccgtaaag ctgcccgact
     3181 accacttcgt ggaccaccgc atcgagatcc tgagccacga caaggactac aacaaggtga
     3241 agctgtacga gcacgccgtg gcccgcaact ccaccgacag catggacgag ctgtacaagg
     3301 gtggcagcgg tggcatggtg agcaagggcg aggagaccat tacaagcgtg atcaagcctg
     3361 acatgaagaa caagctgcgc atggagggca acgtgaacgg ccacgccttc gtgatcgagg
     3421 gcgagggcag cggcaagccc ttcgagggca tccagacgat tgatttggag gtgaaggagg
     3481 gcgccccgct gcccttcgcc tacgacatcc tgaccaccgc cttccactac ggcaaccgcg
     3541 tgttcaccaa gtacccacgg gtcgagccat ctgctggaga catgagagct gccaaccttt
     3601 ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg tccctgacgg
     3661 ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat tccgagtatg
     3721 atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac ctggcagaca
     3781 gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg gatttgaccc
     3841 tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg attggtctcg
     3901 tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg ctcttggaca
     3961 ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg ctggctacat
     4021 catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc aaatctatta
     4081 ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct ctggaagaga
     4141 aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac ctgatggcca
     4201 aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc ctcatcctct
     4261 cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg aagtgcaaga
     4321 acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc
     4381 ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg gccactgcgg
     4441 gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca gagggtttcc
     4501 ctgccacagc ttgatagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
     4561 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     4621 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     4681 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4741 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4801 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     4861 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4921 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4981 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     5041 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     5101 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5161 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5221 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     5281 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5341 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5401 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     5461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5581 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6661 ccccgtcatg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6841 ttgccaggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7561 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
     7621 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7681 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7741 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7801 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7861 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7921 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     7981 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     8041 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg
     8101 gcggggcggg g
//
