LOCUS       sequence-177095-        9851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-4
FEATURES             Location/Qualifiers
     source          1..9851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..161)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(302..961)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     876..895
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(962..1064)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1991..2210
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2301..3056
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3059..3309
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3635..4810
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4810..5781
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5854..6327
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6587..6985
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     primer_bind     complement(7127..7146)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7246..7268
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7306..7324)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7392..7496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7497..7565,7566..8357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7715..7734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8528..9116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9017..9036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9270..9287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9387..9785
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9803..9836)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taatacgact cactataggg agaagcttgc tgaggacact ctttccctac acgacgctct
       61 tccgatcttc atccacgtgc atgctggatc cgatacagat cggaagagca cacgtctgaa
      121 ctccagtcac atcctcagcg atcctatagt gtcacctaaa tcgtatgcgg ccgcccgggc
      181 cgtcgaccaa ttctcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat
      241 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
      301 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      361 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      421 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      481 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
      541 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      601 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
      661 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
      721 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
      781 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
      841 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
      901 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
      961 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1021 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     1081 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     1141 agtgatcttc cgtcacaggt atttattcgc gataagctca tggagcggcg taaccgtcgc
     1201 acaggaagga cagagaaagc gcggatctgg gaagtgacgg acagaacggt caggacctgg
     1261 attggggagg cggttgccgc cgctgctgct gacggtgtga cgttctctgt tccggtcaca
     1321 ccacatacgt tccgccattc ctatgcgatg cacatgctgt atgccggtat accgctgaaa
     1381 gttctgcaaa gcctgatggg acataagtcc atcagttcaa cggaagtcta cacgaaggtt
     1441 tttgcgctgg atgtggctgc ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg
     1501 gttgcgatgc tgaaacaatt atcctgagaa taaatgcctt ggcctttata tggaaatgtg
     1561 gaactgagtg gatatgctgt ttttgtctgt taaacagaga agctggctgt tatccactga
     1621 gaagcgaacg aaacagtcgg gaaaatctcc cattatcgta gagatccgca ttattaatct
     1681 caggagcctg tgtagcgttt ataggaagta gtgttctgtc atgatgcctg caagcggtaa
     1741 cgaaaacgat ttgaatatgc cttcaggaac aatagaaatc ttcgtgcggt gttacgttga
     1801 agtggagcgg attatgtcag caatggacag aacaacctaa tgaacacaga accatgatgt
     1861 ggtctgtcct tttacagcca gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg
     1921 agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca cgatgcctga
     1981 aaaaacttcc cttggggtta tccacttatc cacggggata tttttataat tatttttttt
     2041 atagttttta gatcttcttt tttagagcgc cttgtaggcc tttatccatg ctggttctag
     2101 agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat caccctcaaa tgacagtcct
     2161 gtctgtgaca aattgccctt aaccctgtga caaattgccc tcagaagaag ctgttttttc
     2221 acaaagttat ccctgcttat tgactctttt ttatttagtg tgacaatcta aaaacttgtc
     2281 acacttcaca tggatctgtc atggcggaaa cagcggttat caatcacaag aaacgtaaaa
     2341 atagcccgcg aatcgtccag tcaaacgacc tcactgaggc ggcatatagt ctctcccggg
     2401 atcaaaaacg tatgctgtat ctgttcgttg accagatcag aaaatctgat ggcaccctac
     2461 aggaacatga cggtatctgc gagatccatg ttgctaaata tgctgaaata ttcggattga
     2521 cctctgcgga agccagtaag gatatacggc aggcattgaa gagtttcgcg gggaaggaag
     2581 tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa aggctatgaa tcttttcctt
     2641 ggtttatcaa acgtgcgcac agtccatcca gagggcttta cagtgtacat atcaacccat
     2701 atctcattcc cttctttatc gggttacaga accggtttac gcagtttcgg cttagtgaaa
     2761 caaaagaaat caccaatccg tatgccatgc gtttatacga atccctgtgt cagtatcgta
     2821 agccggatgg ctcaggcatc gtctctctga aaatcgactg gatcatagag cgttaccagc
     2881 tgcctcaaag ttaccagcgt atgcctgact tccgccgccg cttcctgcag gtctgtgtta
     2941 atgagatcaa cagcagaact ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc
     3001 agacgactca tatcgtattt tccttccgcg atatcacttc catgacgaca ggatagtctg
     3061 agggttatct gtcacagatt tgagggtggt tcgtcacatt tgttctgacc tactgagggt
     3121 aatttgtcac agttttgctg tttccttcag cctgcatgga ttttctcata ctttttgaac
     3181 tgtaattttt aaggaagcca aatttgaggg cagtttgtca cagttgattt ccttctcttt
     3241 cccttcgtca tgtgacctga tatcgggggt tagttcgtca tcattgatga gggttgatta
     3301 tcacagttta ttactctgaa ttggctatcc gcgtgtgtac ctctacctgg agtttttccc
     3361 acggtggata tttcttcttg cgctgagcgt aagagctatc tgacagaaca gttcttcttt
     3421 gcttcctcgc cagttcgctc gctatgctcg gttacacggc tgcggcgagc gctagtgata
     3481 ataagtgact gaggtatgtg ctcctcttat ctccttttgt agtgttgctc ttattttaaa
     3541 caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt aaattgcaag
     3601 atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac tcatggaaac
     3661 acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca ttgcacagtt
     3721 taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag gtgaagcagc
     3781 ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag ggcgactacc
     3841 gcacccggat atggaaattc gaggacgggt tgagcaacga gttggttata caattgaaca
     3901 aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag acgtatttcc
     3961 accggtgatc ggggttgctg ctcataaagg tggcgtttac aaaacctcag tttctgttca
     4021 tcttgctcag gatctggctc tgaaggggct acgagttttg ctcgtggaag gtaacgaccc
     4081 ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc atgcagaaga
     4141 cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa taaagcccac
     4201 ttgctggccg gggcttgaca ttattccttc ctgtctggct ctgcaccgta ttgaaactga
     4261 gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga tgctccgact
     4321 ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg cacctaacct
     4381 gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc ccacgcctgc
     4441 tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg atctgctcaa
     4501 gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca aatacagcaa
     4561 tagtaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct ggggaagcat
     4621 ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga tccggatgag
     4681 aactgttttt gaacaggcca ttgatcaacg ctcctcaact ggtgcctgga gaaatgctct
     4741 ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac cacgctggga
     4801 gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac tcaaccggtt
     4861 gaagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat tgcgcgcgta
     4921 ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga tgtgaagttt
     4981 actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg gtcaggtaat
     5041 gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc ttcttttcta
     5101 ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat agaaattgcc
     5161 gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg tgttctggtt
     5221 ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga ttatcgccca
     5281 acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga atttgctgga
     5341 aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac ccgctgtatc
     5401 aacaccgcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg tgaactatct
     5461 gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt acttaagcag
     5521 caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc tgaagaagtt
     5581 atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag tttaagctca
     5641 cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat ggtgcttaac
     5701 ctggacaggt ctcgtgttcc aactgagtgt atagagaaaa ttgaggccat tcttaaggaa
     5761 cttgaaaagc cagcaccctg atgcgaccac gttttagtct acgtttatct gtctttactt
     5821 aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc tgggcccact
     5881 gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac tcgtatcgtc
     5941 ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct
     6001 gggaccacgg tcccactcgt atcgtcggtc tgataatcag actgggacca cggtcccact
     6061 cgtatcgtcg gtctgattat tagtctggga ccatggtccc actcgtatcg tcggtctgat
     6121 tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt ctggaaccac
     6181 ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt
     6241 cggtctgatt attagtctgg gaccacgatc ccactcgtgt tgtcggtctg attatcggtc
     6301 tgggaccacg gtcccacttg tattgtcgat cagactatca gcgtgagact acgattccat
     6361 caatgcctgt caagggcaag tattgacatg tcgtcgtaac ctgtagaacg gagtaacctc
     6421 ggtgtgcggt tgtatgcctg ctgtggattg ctgctgtgtc ctgcttatcc acaacatttt
     6481 gcgcacggtt atgtggacaa aatacctggt tacccaggcc gtgccggcac gttaaccggg
     6541 ctgcatccga tgcaagtgtg tcgctgtcga cgagctcgcg agctcggaca tgaggttgcc
     6601 ccgtattcag tgtcgctgat ttgtattgtc tgaagttgtt tttacgttaa gttgatgcag
     6661 atcaattaat acgatacctg cgtcataatt gattatttga cgtggtttga tggcctccac
     6721 gcacgttgtg atatgtagat gataatcatt atcactttac gggtcctttc cggtgatccg
     6781 acaggttacg gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa
     6841 ggcgtttccg ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag
     6901 aaaggaaacg acaggtgctg aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt
     6961 tttgtccgtg gaatgaacaa tggaagtcct ggcgtaatag cgaagaggcc cgcaccgatc
     7021 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     7081 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     7141 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7201 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     7261 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     7321 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaggagc
     9301 gcccaatacg caaaccgcct ctgtttaaac gttggccgat tcattaatgc agcttgcgcc
     9361 gttccacttc gatgcgtcag tgaagcgaca tgaggcgcgc ccgtattcag tgtcgctgat
     9421 ttgtattgtc tgaagttgtt tttacgttaa gttgaagcag atcaattaat acgatacctg
     9481 cgtcataatt gattatttga cgtggtttga tggcctccac gcacgttgtg atatgtagaa
     9541 gataatcatt atcactttac gggtcctttc cggtgatccg acaggttacg gggcggcgac
     9601 ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg
     9661 tcataactta atgaatttat ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg
     9721 aaagcgagct ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa
     9781 tggaagtccg agctcatcgc taataacttc gtatagcata cattatacga agttatattc
     9841 gatgcggccg c
//
