LOCUS       sequence-177104-        9866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make Fosmids that can be converted into Illumina sequencable 
            libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina.
  JOURNAL   Genome Res. 2012 Nov;22(11):2241-9. doi: 10.1101/gr.138925.112. Epub
            2012 Jul 16.
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.138925.112"; journalName: "Genome Res"; date: "2012-11";
            volume: "22"; issue: "11"; pages: "2241-9"
COMMENT     Sequence Label: pFosill-3
FEATURES             Location/Qualifiers
     source          1..9866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(159..176)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(317..976)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     891..910
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(977..1079)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      2006..2225
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2316..3071
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    3074..3324
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3650..4825
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4825..5796
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5869..6342
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6602..7000
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     primer_bind     complement(7142..7161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7261..7283
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7321..7339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7407..7511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7512..7580,7581..8372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7730..7749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8543..9131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9032..9051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9285..9302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9402..9800
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9818..9851)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 taatacgact cactataggg agaagcttgc tgaggacact ctttccctac acgacgctct
       61 tccgatcttc atccacgtgc atgctggatc ctacctcatt cgtgatacag atcggaagag
      121 cggttcagca ggaatgccga gaccgatcct cagcgatcct atagtgtcac ctaaatcgta
      181 tgcggccgcc cgggccgtcg accaattctc atgtttgaca gcttatcatc gaatttctgc
      241 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
      301 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      361 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      421 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      481 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      541 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      601 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      661 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      721 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
      781 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      841 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      901 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      961 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1021 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1081 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1141 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcgcgataa gctcatggag
     1201 cggcgtaacc gtcgcacagg aaggacagag aaagcgcgga tctgggaagt gacggacaga
     1261 acggtcagga cctggattgg ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc
     1321 tctgttccgg tcacaccaca tacgttccgc cattcctatg cgatgcacat gctgtatgcc
     1381 ggtataccgc tgaaagttct gcaaagcctg atgggacata agtccatcag ttcaacggaa
     1441 gtctacacga aggtttttgc gctggatgtg gctgcccggc accgggtgca gtttgcgatg
     1501 ccggagtctg atgcggttgc gatgctgaaa caattatcct gagaataaat gccttggcct
     1561 ttatatggaa atgtggaact gagtggatat gctgtttttg tctgttaaac agagaagctg
     1621 gctgttatcc actgagaagc gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat
     1681 ccgcattatt aatctcagga gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat
     1741 gcctgcaagc ggtaacgaaa acgatttgaa tatgccttca ggaacaatag aaatcttcgt
     1801 gcggtgttac gttgaagtgg agcggattat gtcagcaatg gacagaacaa cctaatgaac
     1861 acagaaccat gatgtggtct gtccttttac agccagtagt gctcgccgca gtcgagcgac
     1921 agggcgaagc cctcgagtga gcgaggaagc accagggaac agcacttata tattctgctt
     1981 acacacgatg cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt
     2041 ataattattt tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat
     2101 ccatgctggt tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc
     2161 tcaaatgaca gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga
     2221 agaagctgtt ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca
     2281 atctaaaaac ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc
     2341 acaagaaacg taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat
     2401 atagtctctc ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat
     2461 ctgatggcac cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg
     2521 aaatattcgg attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt
     2581 tcgcggggaa ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct
     2641 atgaatcttt tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg
     2701 tacatatcaa cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt
     2761 ttcggcttag tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc
     2821 tgtgtcagta tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca
     2881 tagagcgtta ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc
     2941 tgcaggtctg tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga
     3001 aaaagaaagg ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga
     3061 cgacaggata gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc
     3121 tgacctactg agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc
     3181 tcatactttt tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt
     3241 gatttccttc tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt
     3301 gatgagggtt gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta
     3361 cctggagttt ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca
     3421 gaacagttct tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg
     3481 cgagcgctag tgataataag tgactgaggt atgtgctcct cttatctcct tttgtagtgt
     3541 tgctcttatt ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt
     3601 gcagtaaatt gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat
     3661 gaaactcatg gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat
     3721 cgccattgca cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag
     3781 aataggtgaa gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa
     3841 agcagggcga ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgagttgg
     3901 ttatacaatt gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc
     3961 tgaagacgta tttccaccgg tgatcggggt tgctgctcat aaaggtggcg tttacaaaac
     4021 ctcagtttct gttcatcttg ctcaggatct ggctctgaag gggctacgag ttttgctcgt
     4081 ggaaggtaac gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca
     4141 tattcatgca gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta
     4201 tgcaataaag cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca
     4261 ccgtattgaa actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca
     4321 cctgatgctc cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga
     4381 cagcgcacct aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat
     4441 tgttcccacg cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct
     4501 tcgtgatctg ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct
     4561 taccaaatac agcaatagta atggctctca gtccccgtgg atggaggagc aaattcggga
     4621 tgcctgggga agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg
     4681 tcagatccgg atgagaactg tttttgaaca ggccattgat caacgctcct caactggtgc
     4741 ctggagaaat gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat
     4801 taaaccacgc tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc
     4861 aatactcaac cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg
     4921 ttaattgcgc gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt
     4981 cgggatgtga agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg
     5041 gtatggtcag gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc
     5101 atcccttctt ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt
     5161 gtcatagaaa ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat
     5221 tatcgtgttc tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt
     5281 aacgattatc gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag
     5341 aatgaatttg ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt
     5401 attacccgct gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac
     5461 cccggtgaac tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag
     5521 gaattactta agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt
     5581 gaagctgaag aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga
     5641 actagtttaa gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat
     5701 aaaatggtgc ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag
     5761 gccattctta aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt
     5821 tatctgtctt tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt
     5881 ctctctgggc ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt
     5941 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     6001 tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg
     6061 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg
     6121 tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta
     6181 ttagtctgga accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg
     6241 tcccactcgt atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg
     6301 gtctgattat cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg
     6361 agactacgat tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta
     6421 gaacggagta acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct
     6481 tatccacaac attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc
     6541 ggcacgttaa ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc
     6601 ggacatgagg ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac
     6661 gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg
     6721 tttgatggcc tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc
     6781 ctttccggtg atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa
     6841 aattttccgg tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa
     6901 ataccctctg aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg
     6961 tttcctttct ctgtttttgt ccgtggaatg aacaatggaa gtcctggcgt aatagcgaag
     7021 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     7081 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     7141 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7201 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7261 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7321 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7381 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7441 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7501 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7561 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7621 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7681 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7741 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7801 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7861 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7921 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7981 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8041 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8101 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8161 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8221 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8281 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8341 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8401 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8461 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8521 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8581 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8641 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8701 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8761 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8821 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8881 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8941 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9001 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9061 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9301 aggaagcgga ggagcgccca atacgcaaac cgcctctgtt taaacgttgg ccgattcatt
     9361 aatgcagctt gcgccgttcc acttcgatgc gtcagtgaag cgacatgagg cgcgcccgta
     9421 ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga agcagatcaa
     9481 ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg
     9541 ttgtgatatg tagaagataa tcattatcac tttacgggtc ctttccggtg atccgacagg
     9601 ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt
     9661 ttccgttctt cttcgtcata acttaatgaa tttatttaaa ataccctctg aaaagaaagg
     9721 aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt
     9781 ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata gcatacatta
     9841 tacgaagtta tattcgatgc ggccgc
//
