LOCUS       sequence-177106-        5191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 490 / 553, Emission: 
            507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5191)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..5191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1105..1797
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     polyA_signal    1919..2040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1956..1975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2010..2029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2047..2502)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2184..2205)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2396..2415
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2529..2633
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2635..2992
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2635..2826
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2658..2678)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2843..2978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3027..3821
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3027..3821
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3081..3100)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3691..3710
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4009..4028)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4053..4100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4429..5017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4918..4937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttatg tcgagcaaga gagccaaggc caagaccacc
      601 aagaagaggc cccagagggc tacgtccaat gtcttcgcca tgtttgacca gtcccagatc
      661 caggagttta aggaggcctt caacatgatt gatcagaacc gcgatggctt cattgataag
      721 gaggacctgc acgacatgtt ggcctctctg gggaagaacc ccacagacga gtacctggag
      781 ggcatgatga acgaggcgcc agggcccatc aacttcacca tgttcctcac aatgtttggg
      841 gagaagctga acggcacaga ccccgaggat gtgatccgca atgcctttgc ctgctttgat
      901 gaggaggcct caggcttcat ccacgaggac cacctgaggg agctgctcac caccatgggc
      961 gaccgattca cggatgagga ggtggacgag atgtaccgcg aggcacccat tgataagaag
     1021 ggcaacttca actacgtgga gttcactcgc atcctcaaac acggcgccaa ggacaaggac
     1081 gacctggatc caccggtcgc caccatgaac accccgggaa ttaacctgat caaggaggac
     1141 atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc acgccttcgt gatcgagggc
     1201 gagggcaagg gcaagcccta cgagggcacc cagaccgcca acctgaccgt gaaggagggc
     1261 gcccccctgc ccttcagcta cgacatcctg accaccgccg tgcactacgg caaccgggtg
     1321 ttcaccaagt accccgagga catccccgac tacttcaagc agagcttccc cgagggctac
     1381 agctgggagc gcaccatgac cttcgaggac aagggcatct gcaccatccg cagcgacatc
     1441 agcctggagg gcgactgctt cttccagaac gtgcgcttca agggcaccaa cttccccccc
     1501 aacggccccg tgatgcagaa gaagaccctg aagtgggagc ccagcaccga gaagctgcac
     1561 gtgcgcgacg gcctgctggt gggcaacatc aacatggccc tgctgctgga gggcggcggc
     1621 cactacctgt gcgacttcaa gaccacctac aaggccaaga aggtggtgca gctgcccgac
     1681 gcccacttcg tggaccaccg catcgagatc ctgggcaacg acagcgacta caacaaggtg
     1741 aagctgtacg agcacgccgt ggcccgctac agccccctgc ccagccaggt gtggtaagcg
     1801 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     1861 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     1921 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1981 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2041 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2101 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2161 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2221 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2281 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2341 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2401 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2461 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     2521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     2641 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     2701 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     2761 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     2821 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2881 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     2941 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     3001 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     3061 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3121 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     3181 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     3241 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3301 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3361 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3421 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3481 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3541 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3601 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3661 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3721 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3781 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     3841 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     3901 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     3961 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     4021 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     4081 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     4141 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     4201 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     4261 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4561 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5041 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5101 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
     5161 cattagttca tagcccatat atggagttcc g
//
