LOCUS       sequence-177109-        5977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-SSTR3-N-17
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1894..1903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1900..2577
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     polyA_signal    2705..2826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2742..2761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2796..2815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2833..3288)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2970..2991)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3182..3201
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3315..3419
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3421..3778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3421..3612
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3444..3464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3629..3764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3813..4607
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3813..4607
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3867..3886)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4477..4496
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4795..4814)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4839..4886
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5215..5803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5704..5723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagac cgccatggcc actgttacct atccttcatc tgagcctacg
      601 accttggacc ctgggaacgc atcctcgacc tggcccctgg ataccaccct ggggaacaca
      661 tccgctggcg ctagcctgac aggcctggct gtcagtggca tcttgatctc tctggtgtac
      721 ctggtggtgt gcgtggtggg tttgctgggc aactcgctgg tgatctacgt ggtcctgcgg
      781 cacacgtcca gcccatcagt gaccagtgtc tatatcctca acctggctct ggctgatgag
      841 ctcttcatgc tagggctacc cttcctggct gctcagaacg ccctgtccta ctggcccttc
      901 ggatctctca tgtgccgtct ggtcatggcc gtggatggca tcaaccagtt caccagcatc
      961 ttctgcctca ccgtcatgag tgtggaccgc tatctggctg tggtgcaccc cacacgctca
     1021 gcccgctggc gcacggcacc agtggctcgc acggtcagtg cagctgtctg ggtggcctcg
     1081 gctgtggtgg tgctgcctgt ggttgtgttc tcaggagtgc cccggggcat gagcacgtgc
     1141 cacatgcagt ggccagagcc agcggctgcc tggcgaacag ccttcatcat ctacacggcc
     1201 gcactgggct tctttgggcc cctgctggtc atctgcttgt gctacttgct cattgtggta
     1261 aaggtgcggt cgaccacccg gcgggtgcgg gcgccctcgt gtcagtgggt acaggcaccc
     1321 gcatgccagc ggcgacgccg ctctgagcgc agggtcacac gcatggtggt ggccgtggtg
     1381 gcactcttcg tcctctgctg gatgcctttc tatctgctca acatcgtcaa tgtggtgtgc
     1441 ccgctgccgg aggagcccgc cttcttcggc ctctacttcc tggtggtggc gctgccctac
     1501 gccaacagct gcgcaaaccc catcctctac ggcttcctct cctaccgctt caagcagggc
     1561 tttcgcagga tcctgctaag accatcacgt cgcattcgga gccaggagcc agggtcggga
     1621 cctccagaga agactgaaga ggaggaggat gaagaagaag aagagagaag ggaagaggag
     1681 gagcggagga tgcagagagg gcaggagatg aacgggaggc tcagtcagat cgcacaggct
     1741 ggcactagtg gacaacagcc acggccctgc acagggactg ctaaggagca gcagcttctg
     1801 ccccaggagg ccacagctgg ggacaaggcc agcacactga gccatctggg aattctgcag
     1861 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagtgt gattaaacca
     1921 gacatgaaga tcaagctgcg tatggaaggc gctgtaaatg gacacccgtt cgcgattgaa
     1981 ggagttggcc ttgggaagcc tttcgaggga aaacagagta tggaccttaa agtcaaagaa
     2041 ggcggacctc tgcctttcgc ctatgacatc ttgacaactg tgttctgtta cggcaacagg
     2101 gtattcgcca aatacccaga aaatatagta gactatttca agcagtcgtt tcctgagggc
     2161 tactcttggg aacgaagcat gaattacgaa gacgggggca tttgtaacgc gacaaacgac
     2221 ataaccctgg atggtgactg ttatatctat gaaattcgat ttgatggtgt gaactttcct
     2281 gccaatggtc cagttatgca gaagaggact gtgaaatggg agccatccac tgagaaattg
     2341 tatgtgcgtg atggagtgct gaagggtgat gttaacatgg ctctgtcgct tgaaggaggt
     2401 ggccattacc gatgtgactt caaaactact tataaagcta agaaggttgt ccagttgcca
     2461 gactatcact ttgtggacca ccacattgag attaaaagcc acgacaaaga ttacagtaat
     2521 gttaatctgc atgagcacgc cgaagcgcat tctgagctgc cgaggcaggc caagtaatga
     2581 taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2641 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2701 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2821 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2881 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2941 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3001 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3061 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3121 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3181 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3241 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     3301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     3421 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3601 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3661 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3721 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3781 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3961 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     4021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4321 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4621 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4681 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4741 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4801 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4861 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4921 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4981 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     5041 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     5101 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5881 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     5941 cggggtcatt agttcatagc ccatatatgg agttccg
//
