LOCUS       sequence-177119-       11936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of pHoenix 
            (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I 
            promoter for optogenetic acidification of synaptic vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11936)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 11936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pLenti-Synapsin-pHoenix-WPRE
FEATURES             Location/Qualifiers
     source          1..11936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      762..771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             768..1484
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(801..829)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1424..1444
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1500..2273
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     CDS             2280..2975
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    3747..4335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3800..3820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4218..4229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4338..4354)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4339..4355)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4860..5040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5066..5083)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5072..5296
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5342..5770
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5429..5448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5639..5660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5779..5799)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5784..6113
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5964..6099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6026..6045
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6161..6208
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6227..6601
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6731..6852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6768..6787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6822..6841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6901..6917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6901..6917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6914..6936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6925..6941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6949..6955,6956..6973,6974..6979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6994..7015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7132..7149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7303..7891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7383..7402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8062..8853,8854..8922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8685..8704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8923..9027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9102..9121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9293..9672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9674..9872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9827..9847
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9890..10070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10117..10242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10735..10968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11153..11197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11495..11612
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11681..11936,1..192)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
       61 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
      121 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
      181 ctgagagcgc agtcgctagc gccaccatgg acgtggtgaa tcagctggtg gctgggggtc
      241 agttccgggt ggtcaaggag ccccttggct tcgtgaaggt gctgcagtgg gtctttgcca
      301 tcttcgcctt tgctacgtgc ggcagctaca ccggagagct tcggctgagc gtggagtgtg
      361 ccaacaagac ggagagtgcc ctcaacatcg aagtcgaatt tgagtaccca ttcaggctgc
      421 accaagtgta ctttgatgca ccctcctgcg ttaaaggggg cactaccaag atcttcctag
      481 ttggtgacta ctcctcctcg gctgaattct ttgtcaccgt ggctgtgttt gccttcctct
      541 actccatggg ggccctggcc acctacatct tcctgcagaa caagtaccga gagaacaaca
      601 aagggccaat gatggacttc ctggccacag cagtgttcgc tttcatgtgg ctagttagct
      661 catccgcctg ggccaaaggc ctgtccgatg tgaagatggc cactgaccca gagaacatta
      721 tcaaggagat gcctatgtgc cgccagacag atatggatct agccaccatg ggaagtaaag
      781 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      841 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      901 ttaaatttat ttgcactact ggaaaactac ctgttccttg gccaacactt gtcactactt
      961 taacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg catgactttt
     1021 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1081 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1141 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1201 actataacga tcaccaggtg tacatcatgg cagacaaaca aaagaatgga atcaaagcta
     1261 acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac cattatcaac
     1321 aaaatactcc aattggcgat gggcccgtcc ttttaccaga caaccattac ctgtttacaa
     1381 cttctactct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1441 taacagctgc tgggattaca catggcatgg atgaactata caaaaccggt accggatcca
     1501 tggaccccat cgctctgcag gctggttacg acctgctggg tgacggcaga cctgaaactc
     1561 tgtggctggg catcggcact ctgctgatgc tgattggaac cttctacttt ctggtccgcg
     1621 gatggggagt caccgataag gatgcccggg aatattacgc tgtgactatc ctggtgcccg
     1681 gaatcgcatc cgccgcatat ctgtctatgt tctttggtat cgggcttact gaggtgaccg
     1741 tcgggggcga aatgttggat atctattatg ccaggtacgc cgactggctg tttaccaccc
     1801 cacttctgct gctggatctg gcccttctcg ctaaggtgga tcgggtgacc atcggcaccc
     1861 tggtgggtgt ggacgccctg atgatcgtca ctggcctcat cggagccttg agccacacgg
     1921 ccatagccag atacagttgg tggttgttct ctacaatttg catgatagtg gtgctctatt
     1981 ttctggctac atccctgcga tctgctgcaa aggagcgggg ccccgaggtg gcatctacct
     2041 ttaacaccct gacagctctg gtcttggtgc tgtggaccgc ttaccctatc ctgtggatca
     2101 taggcactga gggcgctggc gtggtgggcc tgggcatcga aactctgctg tttatggtgt
     2161 tggacgtgac tgccaaggtc ggctttggct ttatcctgtt gagatcccgg gctattctgg
     2221 gcgacaccga ggcaccagaa cccagtgccg gtgccgatgt cagtgccgcc gaccgtacga
     2281 tggtgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga
     2341 acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     2401 ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     2461 ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct
     2521 ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac gaagacgggg
     2581 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     2641 agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
     2701 gggaggcctc caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agagccgaca
     2761 tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat
     2821 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg
     2881 aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca
     2941 gatactgcga cctccctagc aaactggggc acagaaagct tgcagccctg caggagaaga
     3001 agtcatgcag ccagcgcatg gccgaattcc ggcaatactg ttggaacccg gacactgggc
     3061 agatgctggg ccgcacccca gcccggtggg tgtggatcag cctgtactat gcagctttct
     3121 acgtggtcat gactgggctc tttgccttgt gcatctatgt gctgatgcag accattgatc
     3181 cctacacccc cgactaccag gaccagttaa agtcaccggg ggtaaccttg agaccggatg
     3241 tgtatgggga aagagggctg cagatttcct acaacatctc tgaaaacagc tctggtttaa
     3301 acaccaccgg tggagctcga atcacatgca aggaactgag ggaccctgtg acttcaggac
     3361 tcaacacctc ggtggtgttt ggcttcctga acctggtgct ctgggttggc aacctatggt
     3421 tcgtgttcaa ggagacaggc tgggccgccc cattcatgcg cgcacctcca ggcgccccag
     3481 aaaagcaacc agctcctggc gatgcctacg gcgatgcggg ctatgggcag ggccccggag
     3541 gctatgggcc ccaggactcc tacgggcctc agggtggtta tcaacccgat tacgggcagc
     3601 cagccagcgg cggtggcggt ggctacgggc ctcagggcga ctatgggcag caaggctacg
     3661 gccaacaggg tgcgcccacc tccttctcca atcagatgta attaattaag gccgcgactc
     3721 tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     3781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3901 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3961 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4021 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4081 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4141 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4201 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4261 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4321 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     4381 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     4441 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     4501 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4561 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     4621 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     4681 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     4741 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     4801 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     4861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5041 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5101 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5161 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5221 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5281 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5341 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5401 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5461 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5521 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5581 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5641 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5701 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5761 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5941 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6001 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6061 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6121 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     6181 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     6241 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     6301 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     6361 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     6421 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     6481 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     6541 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     6601 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6661 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6721 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6841 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     6901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     8701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9061 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     9121 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     9181 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     9241 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     9301 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     9361 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     9421 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     9481 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     9541 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     9601 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     9661 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     9721 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     9781 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     9841 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     9901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10081 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10141 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10201 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10261 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10321 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10381 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10441 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10501 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10561 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10621 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10681 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10741 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10801 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10861 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10921 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10981 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    11041 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    11101 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11161 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11221 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11281 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11341 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    11401 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
    11461 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    11521 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    11581 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    11641 cagtttggtt aatcgcatgc tagagagggc cctgcgtatg agtgcaagtg ggttttagga
    11701 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
    11761 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
    11821 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
    11881 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgcc
//
