LOCUS       sequence-17716-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  TITLE     Vectors
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 401 pSG5L
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    47..168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(84..103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     138..157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(202..219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(453..472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttccccgata tcgcctcgag tctagatcca gatcttatta aagcagaact tgtttattgc
       61 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      121 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggtc
      181 gactctagac tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      721 agcagccact ggtaa
//
