LOCUS       sequence-177179-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  envelope pseudotyping of lentiviral vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Schoderboeck L, Riad S, Bokor AM, Wicky HE, Strauss M, Bostina M, 
            Oswald MJ, Empson RM, Hughes SM
  TITLE     Chimeric rabies SADB19-VSVg-pseudotyped lentiviral vectors mediate 
            long-range retrograde transduction from the mouse spinal cord.
  JOURNAL   Gene Ther. 2015 May;22(5):357-64. doi: 10.1038/gt.2015.3. Epub 2015 
            Jan 29.
  PUBMED    25630949
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/gt.2015.3";
            journalName: "Gene Ther"; date: "2015-05"; volume: "22"; issue: "5";
            pages: "357-64"
COMMENT     Sequence Label: B19/VSVg
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1495..1527
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     polyA_signal    1619..2013
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(2352..2369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2523..3111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2603..2622)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3282..4073,4074..4142))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3905..3924
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4143..4247)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4317..4336)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        4438..4817
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4818..5021
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4972..4992
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5018..5042
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5155..5630
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 atggttcctc aggctctcct gtttgtaccc cttctggttt ttccattgtg ttttgggaaa
       61 ttccctattt acacgatacc agacaagctt ggtccctgga gtccgattga catacatcac
      121 ctcagctgcc caaacaattt ggtagtggag gacgaaggat gcaccaacct gtcagggttc
      181 tcctacatgg aacttaaagt tggatacatc ttagccataa aagtgaacgg gttcacttgc
      241 acaggcgttg tgacggaggc tgaaacctac actaacttcg ttggttatgt cacaaccacg
      301 ttcaaaagaa agcatttccg cccaacacca gatgcatgta gagccgcgta caactggaag
      361 atggccggtg accccagata tgaagagtct ctacacaatc cgtaccctga ctaccgctgg
      421 cttcgaactg taaaaaccac caaggagtct ctcgttatca tatctccaag tgtggcagat
      481 ttggacccat atgacagatc ccttcactcg agggtcttcc ctagcgggaa gtgctcagga
      541 gtagcggtgt cttctaccta ctgctccact aaccacgatt acaccatttg gatgcccgag
      601 aatccgagac tagggatgtc ttgtgacatt tttaccaata gtagagggaa gagagcatcc
      661 aaagggagtg agacttgcgg ctttgtagat gaaagaggcc tatataagtc tttaaaagga
      721 gcatgcaaac tcaagttatg tggagttcta ggacttagac ttatggatgg aacatgggtc
      781 tcgatgcaaa catcaaatga aaccaaatgg tgccctcccg ataagttggt gaacctgcac
      841 gactttcgct cagacgaaat tgagcacctt gttgtagagg agttggtcag gaagagagag
      901 gagtgtctgg atgcactaga gtccatcatg acaaccaagt cagtgagttt cagacgtctc
      961 agtcatttaa gaaaacttgt ccctgggttt ggaaaagcat ataccatatt caacaagacc
     1021 ttgatggaag ccgatgctca ctacaagtca gtcagaactt ggaatgagat cctcccttca
     1081 aaagggtgtt taagagttgg ggggaggtgt catcctcatg tgaacggggt gtttttcaat
     1141 ggtataatat taggacctga cggcaatgtc ttaatcccag agatgcaatc atccctcctc
     1201 cagcaacata tggagttgtt ggaatcctcg gttatccccc ttgtgcaccc cctggcagac
     1261 ccgtctaccg ttttcaagga cggtgacgag gctgaggatt ttgttgaagt tcaccttccc
     1321 gatgtgcaca atcaggtctc aggagttgac ttgggtctcc cgaactgggg gaagtatgta
     1381 ttactgagtg caggggccct gactgccttg atgttgatag ttttcctgat gacatgttgt
     1441 cgagttggta tccatctttg cattaaatta aagcacacca agaaaagaca gatttataca
     1501 gacatagaga tgaaccgact tggaaagtaa atcgtcggaa ttcaccccac cagtgcaggc
     1561 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
     1621 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
     1681 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
     1741 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
     1801 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
     1861 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
     1921 cagcccctga tgcctatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
     1981 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
     2041 tgaatgtctt ttcactaccc atttgcttat cctgcatctc tcagccttga ctccactcag
     2101 ttctcttgct tagagatacc acctttcccc tgaagtgttc cttccatgtt ttacggcgag
     2161 atggtttctc ctcgcctggc cactcagcct tagttgtctc tgttgtctta tagaggtcta
     2221 cttgaagaag gaaaaacagg gggcatggtt tgactgtcct gtgagccctt cttccctgcc
     2281 tcccccactc acagtgaccc ggaatccctc gacatggcag tctagcacta gtgcggccgc
     2341 agatctgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2401 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2461 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2521 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2581 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2641 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2701 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2761 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2821 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2881 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2941 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3001 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3061 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3121 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3181 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3241 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3301 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3361 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3421 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3481 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3541 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3601 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3661 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3721 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3781 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3841 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3901 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3961 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4021 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4081 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4141 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4201 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4261 aaagtgccac ctgacgtgga tcccctgagg gggcccccat gggctagagg atccggcctc
     4321 ggcctctgca taaataaaaa aaattagtca gccatgagct tggcccattg catacgttgt
     4381 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
     4441 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     4501 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4561 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4621 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4681 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4741 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     4801 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     4861 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4921 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4981 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     5041 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     5101 gcctcccctc gaagcttaca tgtggtaccg agctcggatc ctgagaactt cagggtgagt
     5161 ctatgggacc cttgatgttt tctttcccct tcttttctat ggttaagttc atgtcatagg
     5221 aaggggagaa gtaacagggt acacatattg accaaatcag ggtaattttg catttgtaat
     5281 tttaaaaaat gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc
     5341 cctaatctct ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc
     5401 taaagaataa cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata
     5461 tttctgcata taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat
     5521 ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa
     5581 gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca
     5641 acgtgctggt ctgtgtgctg gcccatcact ttggcaaagc acgtgagatc tgaattcgcc
     5701 cttatagatc tccgcggtga cact
//
