LOCUS       sequence-177180-        7907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible Cas9 in a broad host range backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  TITLE     pX2-Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX2-Cas9
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             529..1194
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     CDS             1284..2099
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1357..1376)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(2116..2147)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2175..3053)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3080..3364
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3441..7547
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3441..7544
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      complement(7622..7658)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      join(7664..7907,1..528)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 agtggaaggc gggtcaaggc tcgcgcagcg accgcgcagc ggcttggcct tgacgcgcct
       61 ggaacgaccc aagcctatgc gagtgggggc agtcgaaggg cgaagcccgc ccgcctgccc
      121 cccgagcctc acggcggcga gtgcgggggt tccaaggggg cagcgccacc ttgggcaagg
      181 ccgaaggccg cgcagtcgat caacaagccc cggaggggcc actttttgcc ggagggggag
      241 ccgcgccgaa ggcgtggggg aaccccgcag gggtgccctt ctttgggcac caaagaacta
      301 gatatagggc gaaatgcgaa agacttaaaa atcaacaact taaaaaaggg gggtacgcaa
      361 cagctcattg cggcaccccc cgcaatagct cattgcgtag gttaaagaaa atctgtaatt
      421 gactgccact tttacgcaac gcataattgt tgtcgcgctg ccgaaaagtt gcagctgatt
      481 gcgcatggtg ccgcaaccgt gcggcacccc taccgcatgg agataagcat ggccacgcag
      541 tccagagaaa tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc
      601 aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg
      661 catcacctcg tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt
      721 tccaagctca tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc
      781 gagcgctgga tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc
      841 aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc
      901 gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc
      961 cgcatcccga ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg
     1021 cccagccagc ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag
     1081 gaatgggaac gacgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac
     1141 gatggcgagc cgttggagcc gccgacacgg gtcacgctgc cgcgccgtac gtaagaggtt
     1201 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     1261 tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc
     1321 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1381 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     1441 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcaggctaa
     1501 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1561 atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat
     1621 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1681 cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc
     1741 aatcacgaat gaataacggt ttggttggtg cgagtgattt tgatgacgag cgtaatggct
     1801 ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag
     1861 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1921 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1981 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2041 ttgataatcc tgatatgaat aaattgcagt ttcacttgat gctcgatgag tttttctgag
     2101 ggcggatccc cctcaagtca aaagcctccg gtcggaggtt tttgactttc tgctatggag
     2161 gtcaggtatg atttttatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     2221 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     2281 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     2341 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     2401 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     2461 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     2521 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     2581 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     2641 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     2701 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     2761 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     2821 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     2881 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     2941 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     3001 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     3061 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     3121 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt
     3181 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     3241 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc cataacattt
     3301 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     3361 ccatacccgt ttttttgggc tagcaccgcc tatctcgtgt gagataggcg gagatacgaa
     3421 ctttaagaag gagatatacc atggataaga aatactcaat aggcttagat atcggcacaa
     3481 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
     3541 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
     3601 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
     3661 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
     3721 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
     3781 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
     3841 ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa
     3901 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt
     3961 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc
     4021 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg
     4081 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga
     4141 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat
     4201 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg
     4261 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta
     4321 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta
     4381 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc
     4441 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc
     4501 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata
     4561 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa
     4621 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa
     4681 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa
     4741 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc
     4801 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta
     4861 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac
     4921 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc
     4981 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa
     5041 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca
     5101 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat
     5161 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct
     5221 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag
     5281 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg
     5341 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc
     5401 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc
     5461 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta
     5521 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt
     5581 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta
     5641 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc
     5701 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc
     5761 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc
     5821 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct
     5881 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa
     5941 gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca
     6001 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag
     6061 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc
     6121 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag
     6181 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa
     6241 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta
     6301 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata
     6361 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg
     6421 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata
     6481 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg
     6541 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa
     6601 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct
     6661 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata
     6721 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa
     6781 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt
     6841 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat
     6901 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct
     6961 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact
     7021 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc
     7081 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga
     7141 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac
     7201 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca
     7261 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat
     7321 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta
     7381 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta
     7441 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg
     7501 gtctttatga aacacgcatt gatttgagtc agctaggagg tgactgatct agagaattcg
     7561 tcaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg taactggact
     7621 gaaaacaaac taaagcgccc ttgtggcgct ttagttttgt tccgcggcca ccggctggct
     7681 cgcttcgctc ggcccgtgga caaccctgct ggacaagctg atggacaggc tgcgcctgcc
     7741 cacgagcttg accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg
     7801 gctccaactg cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa
     7861 agcctccggc ctgcggcctg cgcgcttcgc ttgccggttg gacacca
//
