LOCUS       sequence-17719-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression empty backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  TITLE     Vectors
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1435 pSG5L Flag HA
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..53
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             27..53
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    124..245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(161..180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     215..234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(279..296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(530..549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgacgatga caagctcgat ggaggatacc cctacgacgt gcccgactac gccagcggat
       61 ccgcgcggat ccgcgaattc cccgatatcg cctcgagtct agatccagat cttattaaag
      121 cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      241 tcttatcatg tctggtcgac tctagactct tccgcttcct cgctcactga ctcgctgcgc
      301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtang
      661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agt
//
