LOCUS       sequence-177202-        9964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing the fusion protein HA-NFAT1 
            (4-460)-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Leoni C, Montagner S, Rinaldi A, Bertoni F, Polletti S, Balestrieri 
            C, Monticelli S
  TITLE     Dnmt3a restrains mast cell inflammatory responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114.
  PUBMED    28167789
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 6. pii: 201616420. doi: 
            10.1073/pnas.1616420114."
COMMENT     Sequence Label: pSCALPs-HA-NFAT1 (4-460)-GFP
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     179..198
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     438..459
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1915..2004)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(2037..2444)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    2581..2698
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2996..3040)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3224..3457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3954..4079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4126..4306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4324..4522)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4349..4369)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4524..4903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(4915..5244)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4929..5064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4983..5002)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5229..5249
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     5423..5442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5517..5621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5622..5690,5691..6482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5840..5859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6653..7241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7142..7161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7312..7492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8010..8598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8116..8127
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8525..8545
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8608..9207)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8691..8711)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9188..9207
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(join(9749..9964,1..498,499..501,502..504))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9752..9964,1..498,499..501,502..504))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9794..9815)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
       61 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      121 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      181 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      241 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
      301 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      361 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
      421 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
      481 cagctcctcg cccttgctca ccatggtggc gaccggtgga tcgatctgaa gccccagagg
      541 cttgttctcc atgtagccgt ggagctgcac cacagggtgt cctcctgttg gggctttgac
      601 agcgccacgg ctgccctccg tctcatagtg ggcccggtga tggggcttgg gttggacctc
      661 aatccgtagc tcataggagc ccgactgatt ggagagtggc cactcgagtg gtgggaggga
      721 tgcagtcaca gggatgctgc agatgggaat ggccggcacc aactgcttgg gccaagtagg
      781 tggtaccagc aggatggact ctggagcgga attcctcctc tcctcctgct cacatggccc
      841 caggaactcc acagtagggt agatgtggcg ggccaggcca gccttggacg gagcggtgga
      901 cacaggcgtc gggtcaggac tggtcttcca tatcttggag gggatcccgc agggcgagtc
      961 ggtggccagg gtgttgaggg catccatgag aacagcagag ccggccgtgg gggggtaccc
     1021 agcggggatg ctgtcgtcct gcggtgccac gtgaggcgag ggctgtggcg aggggctccg
     1081 ggagcgctgg ggtgaggctg cgggcagagg agcaaccaaa gcctctgcgc acgaatgcct
     1141 ccgcttggca ccgggtgagg aggagcggga tgccggacgg ggcacgggcg agtgtcggcc
     1201 caggcagctg tcctcggcga ggctggttcg aggtgacatt attggagagg ttctggggga
     1261 ataatgagca gggatgtttt gaaactgggg acacaggtcg tcgggcccgg cgttattggg
     1321 tgagacgcag ggcgaggtgt agggggagaa ggtgtcagaa atgaagctgg cagaggagcc
     1381 gctgctagcg gggctcaagc aaagcggctc gcggtagccc tcgtagccgg gcacgggcag
     1441 tgtgaacctc gggctggcgg ccacgccggc cagggccagg gccggctgct cgggggacag
     1501 gccggcgtct ctcccacgga gggccccccc tgcctgcatc agttcgtggg atggagtgat
     1561 ctcgatccga gggctcgggc ccgaagcccc tgctggcttg accgggctca gaaagttctg
     1621 gaaccctata ctatccggct ctccgaaccg gccagggggc tcgccagagg gactggcaag
     1681 ggggttgcat ggcttgaggc catagtccag gacatcatcc gggtatgcga gtccggaggg
     1741 tgagctgatg gccttatgtg cgatcggttc ttcttcgata gggttcagat agtcataatc
     1801 gaagaggatg gaaaagtcca gctcgtcttg gggactgccc ccgggctcgt ggccggggcc
     1861 gtccccgcca tcggggtcgg gctgcggctc cgggctagag cggccgcact gagcagcgta
     1921 atctggaacg tcatatggat aggatcctgc atagtccggg acgtcatagg gatagcccgc
     1981 atagtcagga acatcgtatg ggtaaaagat catggtggaa gcttgagctc tagacccccg
     2041 ggcgactcag tctgtcggag gactggcgcg ccgagtgagg ggttgtgagc tcttttatag
     2101 agctcgggaa gcagaagcgc gcgaacagaa gcgagaagca ggctgattgg ttaattcaaa
     2161 taaggcgcag ggtcatttca ggtccttggg ggagcctgga aacatctgat gggtcttaag
     2221 aaactgctga gggttgggcc atatctgggg accatctgtt cttggcctcg ggccggggcc
     2281 gaaactgcgg tgaccatctg ttcttggccc cgggccgggg ccgaaactgc tcaccgcaga
     2341 tatcctgttt ggcccaacgt tagctatttt catgtacccg cccttgatct gaacttctct
     2401 attcttggtt tggtattttt ccatgccttg caaaatggcg ttactgcagc tagcttgcca
     2461 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
     2521 tttatcgggg ctgcagttaa ttaaccaaac tggatctctg ctgtccctgt aataaacccg
     2581 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     2641 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     2701 gtggatgaat actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta
     2761 atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt
     2821 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
     2881 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
     2941 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
     3001 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
     3061 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat tctctgtccc actccatcca
     3121 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     3181 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     3241 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     3301 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     3361 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     3421 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     3481 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     3541 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     3601 gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc
     3661 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     3721 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     3781 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat
     3841 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     3901 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     3961 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     4021 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     4081 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     4141 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4201 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     4261 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac
     4321 gcgctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     4381 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     4441 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     4501 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     4561 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     4621 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     4681 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     4741 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     4801 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     4861 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcga gctttttgca
     4921 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggcagaggc
     4981 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     5041 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     5101 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     5161 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     5221 ctttccacac cctaactgac acacattcca cagcgcgtat atctggcccg tacatcgcga
     5281 agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc
     5341 cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg
     5401 agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg
     5461 ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     5521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gaaacgggcc ctgctagaga
     7321 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     7381 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     7441 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt
     7501 ccggatgcag ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     7561 atacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt
     7621 tctctccttc attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc
     7681 atccaaaggt cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga
     7741 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     7801 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     7861 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     7921 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     7981 catgtttttc taggtctcga cgcgattaag cggggaggcg gcccaaaggg agatccgact
     8041 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     8101 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     8161 ccaggtggca acacaggcga gcagccatgg aaaggacgtc agcttccccg acaacaccac
     8221 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     8281 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     8341 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     8401 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     8461 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     8521 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     8581 gtaatccaga ggttgattag cgatcgctca ggcaccgggc ttgcgggtca tgcaccaggt
     8641 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     8701 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     8761 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     8821 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     8881 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     8941 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     9001 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     9061 cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta
     9121 gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag
     9181 gcgcaccgtg ggcttgtact cggtcatggt ggcacgcgtg gctaatagta cacggttttc
     9241 ctcggcggtg gcgtctgcaa aacggcacga gcctggcctc ccgccccttt tataccacgt
     9301 tcggccccgc ccgcaacccg gtctcgcgcc tccgtcccgg ccaatcgggt ctgcgacttc
     9361 gccagagtga cagtgcatct ggcctataga cggaagccgg cgctgatggg gacgacacat
     9421 gaggcgcgcg tgcgcgctaa cgcgcggagg caggttcccg cctgcgggct ccgtcgcggt
     9481 ggctgcggca tgggactcgg ggcggggcgg cagtacggga cgcggggcac gggggagggt
     9541 cagtgcggaa cgggggtggg cagcatggcg cgcgcacgcg ggagacggaa gcgcggcggc
     9601 tcttcggccg ttgtcaagcg atgaggcgcg tctccccatt gcacagaggg ggcaactgag
     9661 gcgaacgcag gcctcaaggt cgcgccgcca gtcaaggtcg agaattctgg ccagttatgg
     9721 ctgattgtta actagagtcg cggccgcttt acttgtacag ctcgtccatg ccgagagtga
     9781 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     9841 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     9901 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     9961 ggcg
//
