LOCUS       sequence-177206-        7750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid 2 to prepare Penn State DNA molecular 
            weight ladders.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Henrici RC, Pecen TJ, Johnston JL, Tan S
  TITLE     The pPSU Plasmids for Generating DNA Molecular Weight Markers.
  JOURNAL   Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
  PUBMED    28550309
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-05-26"; volume: "7(1):2438. doi"; pages: " 
            10.1038/s41598-017-02693-1"
COMMENT     Sequence Label: pPSU2
FEATURES             Location/Qualifiers
     source          1..7750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..52)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(286..305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1998..2016
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2054..2076)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2176..2195
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2389..2411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2403..2420
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2404..2420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7554..7570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7554..7570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7567..7589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7578..7594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7602..7608,7609..7626,7627..7632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7647..7668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2041 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2101 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2161 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2341 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2401 gttgtaaaac gacggccagt gaattctcat agatggtcgg tgggaggtgg tacaaattct
     2461 ctcatgcaaa aaatatgtaa aatcggtagc aactggaaat cattcaacac ccgcactatc
     2521 ggaagttcac cagccagcca cagcacgttc ctgcatacga cgtgtctgcg gctctaccat
     2581 atctcctatg agcaacgtgt tagcagagcc aagccacaac tctaatttta atacataatg
     2641 aatgataata ataatattaa aaatttcctg tgtaactaat ttactatatg gtttctgata
     2701 agaatcattg caaagatcaa acaacttgta ttacattgac agttaagcag ttaattttat
     2761 cacctctaaa atatatcagc atctagcatg caacctatca aaatggagag ttttatgact
     2821 aaaaaaccat gggaaagaag acttaaagat ttatcgcact tgctcaaatg ctgcattgat
     2881 acatattttg accctgaatt atttcgcttg aatttgaatc aattcctcca aaccgcaaga
     2941 acagtaacat ttattattca aaaaaacaaa aaccagatta taggatatga catttggtat
     3001 aacaataatg ttattgaaaa atggaaaaat gatccattaa tggcttgggc taaaaattct
     3061 cgcaatacga tagaaaaaca aggcgattta gaaatgtata gcgaggcaaa ggctactctt
     3121 atttcatctt acattgaaga aaatgacatt gagtttatta caaatgaaag tatgttaaac
     3181 attggtataa aaaagttagt cagacttgca caaaagaaat taccttcata tttaactgaa
     3241 tcatctatta ttaaatcaga aagacgatgg gtcgctaata cgctaaaaga ttacgaatta
     3301 ttacatgcct tagctataat ctatggcaga atgtataact gctgtaactc tcttggcata
     3361 caaataaaca atccaatggg tgacgatgtg atttcgccaa catcattcga ctctttattt
     3421 gatgaagcca ggagaataac ttatttaaaa ttaaaagatt actccataag caaattgtca
     3481 tttagcatga tacaatatga caataaaata attcctgaag atattaaaga gcgtctaaaa
     3541 ctggtagata agcctaaaaa tatcacttcg acagaagagt tagttgacta tacagccaag
     3601 cttgcagaaa cgactttttt aaaggacggt tatcacattc aaacattaat tttttatgat
     3661 aaacaattcc atccaattga tttaatcaat acaacatttg aagatcaagc agataaatat
     3721 attttttggc gttatgcagc tgacagagcc aaaataacaa atgcctatgg cttcatttgg
     3781 atatctgtac actgcagatg tcccagcgcc gcaccgctgt cattaatctg cacaccggca
     3841 cggcagttcc ggctgtcgcc ggtattgttc gggttgctga tgcgcttcgg gctgaccatc
     3901 cggaactgtg tccggaaaag ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt
     3961 tcaccgttaa aggcgtgcat ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt
     4021 cgctcaacgt caatgcagca gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc
     4081 tcgcgggaaa aggcacgggc ttcttcctcc ccgatgccca gatagcgcca gcttgggcga
     4141 tgactgagcc ggaaaaaaga cccgacgata tgatcctgat gcagctggat ggcgttggcg
     4201 gcatagccgt tattgcgtac cagatcgtct gcgcgggcat tgccacgggt aaagttgggc
     4261 aacagggctg catccacact ttcactcggt gggttccacg accgcaactg ccctccaaat
     4321 ccgctgccac cgccgtgata accggcatat tcgcgcagcg atgtcatgcc gtccggcccc
     4381 agaagggatc cctgcagttc aggcgctgtt ccgtggcttc ccgttctgcc tgccggtcag
     4441 tcagcccccg gcttttcgca tcaatggcgg cccgttttgc ccgttgctgc tgtgcgaatt
     4501 tatccgcctg ctgcgccagc gcgttcaggc gctcctgata cgtaaccttg tcgccaagtg
     4561 cagccagctg gcgtttgtac tccagcgtct catctttatg cgccagcagg gatttctcct
     4621 gtgcagacag ctggcgacgt tgcgccgcct cctccagtac cgcgaactga ctctccgcct
     4681 tccacaaatc ccggcgctgc tggctgattt tctcatttgc tccgacatgc ttctccagcg
     4741 tccggagttc tgcctgaagc gtcagcaggg cagcatgagc actgtcttcc tgacgatcgc
     4801 ccgcagacac cttcacgctg gactgtttcg gctttttcag cgtcgcttca taatcctttt
     4861 tcgccgccgc catcagcgtg ttgtaatccg cctgcagttt ttgaggactc tgcggctgcg
     4921 gcactttttt ccgcttcagt ggcctttgct gatgccgctt ctgcgccgga ggacgcttcc
     4981 tgagctgacg atgcagcctg tccggcggac gtgctggcgg cgcgtgctga gtcagttgca
     5041 tcagtcacaa gggccgcgac ctgagcagct gatgcactgg catcgccggc tgatttcttc
     5101 gcgtctgccg tactctgtgc caccacggac gcgttacgcg ccacctcttc caccatcagt
     5161 tcaagacgac gcagcacctc cggccgggca tcatcctccg tcatggcaca gagaaaatca
     5221 ttcagcgtcc ccggttgtga atcttcatac acggtgatgg tcccggcgtg cgatggtgga
     5281 aaaccgtcaa cctgcagaga tctggcttcc tgaaaactga agcgcgcttt tgaaggtaac
     5341 gtcaccacgc ggcgaacgat ggcctcttcc agccagcaca gaaacatctg gctcgcctga
     5401 cgggatgcga cgaattttcg ccgccccata aagtacgccc acgactcgtt cgcactggcc
     5461 cgtgccgtgg agtagctcat ctgggcgtaa ttccgggaaa gctgctcata cgagacaccc
     5521 agcccggcag cgatataccg cagcagtgac tgctcaaaca cggagtagcc gttatccgta
     5581 tcctgagccg tctgcagtga cggaatgtta attctcgttg accctgagca ggctgttgag
     5641 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc
     5701 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc
     5761 aatgagagtt gttccgttgt ggggaaagtt actgcaggca agttcttcct gtcgcgtcat
     5821 tcatcctctc cggataaggc acgggcgtaa tctgccagtg ttttcttgtt ggttgctgca
     5881 ccatcctctt cctgcaggcc ggctaaagaa caacctgacc cagcaaaagg tacacaatac
     5941 tctgcagttg gactcaagaa tgctgccagc aatagtttaa aatcactagg cgatctccgc
     6001 ttagaattca tttcagcatt tattggttgt atgagagtag atagaaaaag acaactctgg
     6061 cttgaagcta tcaaaaaact aagtagtgat gaaaactttt caaatatgga actcatcagc
     6121 ctcatttcta aatatgaaga gttaagacgt aatgaaccac agattcaagt ggacgatgat
     6181 aaattcacta aattgtttta tgacaatatc cagaaatatc tgcttcgaat gagctctgga
     6241 catgcaattg ttttatttac tatcacaaga ttagtagatg tcgttggcga aaagtcatta
     6301 gttttattcg atgaaccaga ggttcatctg catccacctt tgctctctgc ttttttacga
     6361 acattaagcg acttactcga tgcacgcaat ggtgtagcaa taattgcaac tcattcccca
     6421 gtagtactgc aagaggttcc aaaatcctgc atgtggaaag tcctacggtc aagagaagca
     6481 ataaatatta tccgtccgga tattgagaca ttcggtgaga acttaggtgt tttaactcgt
     6541 gaggtgtttt tacttgaagt gacaaattct ggataccacc acttattatc gcagtccgtt
     6601 gattcagagc tttcttatga aaccattcta aaaaattata atggtcagat aggattagaa
     6661 ggtcgaaccg ttttaaaagc gatgataatg aacagagatg aaggtaaagt acaatgaaaa
     6721 aactacctct tccagcgaga acttatagcg aaatgcttaa taaatgctcg gaaagatccg
     6781 atatcgcttc actggctgca cgctcaatct gacaggtgcc tctcactttt ggcgtggagc
     6841 cacggatgga ctttggccag accgagcctt caatacgctt gtcacccagg aacgtcggag
     6901 agccttcctg ttcaatatca tcatcaaaag cagcaagttc atcataaccc gccacatcca
     6961 ccgacttttc acggtagttt tttgccgctt taccgcccag gcaccagaag ccacgcccat
     7021 tagtgaaacg cttcatggtg agcgtgttat cccggtgctt tttgccatac cacggggcca
     7081 gcgccagcag cgacggaata tcacgaatag tcggctcaac gtgggttttc ataaagttct
     7141 cggcatcacc atccgttggc aaccagataa gggtgttgcg ctgcttatgc tctataaagt
     7201 aggcataaac acccagcagc attttggaat aaccgacacg ggcagacttc accacattca
     7261 cctcacggat gtagtcgctg cccatcgcat tcatgatggc ccgctgaaag ggcagtgttt
     7321 cccagcgccc ttcctggtat gcggattctt tcgggagata gtaattagca tccgcccatt
     7381 caacggcggt ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc
     7441 gctgcaggtt aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc
     7501 agcgcggctg ctttgttcat ggctttgatg atatcaagct tggcgtaatc atggtcatag
     7561 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7621 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7681 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7741 cgcgcgggga
//
