LOCUS       sequence-177215-        9015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). 
            Auxin-inducible degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
FEATURES             Location/Qualifiers
     source          1..9015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        3243..3622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3625..3900
                     /label=chicken beta-actin promoter
     intron          3901..4909
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4833..4855
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4917..4936
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4988..6712
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             6713..6754
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6764..6817
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6818..7417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6818..6837)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(7445..7462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7451..7658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8621..8638)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8621..8637)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8630..8652)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8846..8865)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8965..8987
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccatttaa atgaaaagtg gagaggcgtg agtcggagaa ctaagaagaa
     2281 acgcctcctc cagggctgag ctccgcccct aggctggcgt gctgcgtact cccccagtac
     2341 tcttgggggt gaggcgtttg tcagaaggag gcgctcagct actttataca cccggaggag
     2401 ggctggctcc gcccccagga gggacggtta ctagcagaat gcagacggtg ctctgcatct
     2461 ttttgtcaag ggacggtgtt ctgatccacg aaaccctccc tcggagagtt cgggcccctc
     2521 cgggccagtg tcctgcaaac aacctctcct gagcttgagg aaagacaaga accgtttcca
     2581 aacccaggtg attcggccac gcccccaagt taaggttccg ccctgtacgt tcccaggtaa
     2641 gctccgcctt gatcccgtcc cactggtaag aaaaggccct gtgataggtg tgctctggtt
     2701 tgcaggctcg cacccagctg gggccccttc ccatgctgct ctgggataaa ggcttttcaa
     2761 gccgcgctcc agagcttggc cttcacaaag aacttcaagg cagtaacttt aattctttag
     2821 cttctccact aggacctctc cttttcacta gttaactctc aggtccccca gtgaactcac
     2881 agtggccagt cacttgttca cttttgctgg gcctcttaga actctaattt acgtaccccc
     2941 tcctgggcct ccgcaagact cacaaaagac tcaacccttt cccaagtcat ccctcccatc
     3001 taatggtgca agcacacaat ctcaggcagc caggacccac aaagtgaaga gtaaaaacgc
     3061 actctgctgg gcagtggtgg cacacacctt taatcccgat gctcaggagg cagaggctgg
     3121 cagtccttga gttccaggcc agcctggtct acagagtgag ttctaggaca gccaggacta
     3181 cactgaaaaa ccctatctca aaacaaacag aaaacatccc aaaatttaaa tggcgcgccg
     3241 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3421 gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca
     3481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3601 attagtcatc gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca
     3661 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     3721 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     3781 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     3841 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     3901 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc
     3961 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     4021 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc
     4081 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt
     4141 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc
     4201 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg
     4261 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     4321 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc
     4381 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg
     4441 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     4501 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg
     4561 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     4621 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct
     4681 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     4741 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga
     4801 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     4861 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     4921 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgaga attctcacgc
     4981 gtccaccatg acatacttcc ctgaggaggt ggtcgaacat attttctcat tccttcccgc
     5041 ccaacgagat aggaataccg tgtccctggt gtgcaaggtc tggtatgaga tcgaacgctt
     5101 gagccgcaga ggtgtttttg tgggtaattg ttatgccgta cgagccgggc gagtggccgc
     5161 ccggttcccg aacgtcaggg cccttacagt gaagggcaag cctcactttg ccgacttcaa
     5221 tttggtgcct ccggactggg gcggctacgc cgggccctgg atcgaggccg ctgcgcgcgg
     5281 gtgtcatggt ctggaagaac tcaggatgaa aaggatggtg gtgtcagacg agtcactgga
     5341 actgcttgcc cgcagcttcc cccgattccg cgccctggtg ctgatcagct gtgaaggatt
     5401 tagcaccgac ggtctggctg ctgtagctag ccattgtaaa ctgctgcgag aactggacct
     5461 gcaggaaaat gaggtagagg ataggggacc tcggtggctg agctgtttcc cggatagctg
     5521 cactagcctg gtgagcttga atttcgcttg tatcaagggc gaagtgaatg ccgggagttt
     5581 ggagagactc gtttcccgat cccccaacct gagatctctg cggctgaaca gatccgtttc
     5641 tgtggacacg ctcgccaaga ttttgctccg gactcccaac ctggaggatc tggggaccgg
     5701 aaatctgacc gatgatttcc agacagagag ctactttaag ctcactagtg ctctggaaaa
     5761 atgcaaaatg cttaggtcac tgagcgggtt ttgggacgct agccctgtct gcctgtcttt
     5821 tatctatccc ctgtgtgcac agttgaccgg acttaatctg agttacgctc caaccctcga
     5881 cgctagtgac cttactaaga tgatcagccg ctgcgtgaag ctccaaaggc tctgggtact
     5941 ggattgtatt tcagacaagg gactgcaagt tgtggcctcc tcctgcaaag atctgcagga
     6001 gctgagggtg ttcccttcag atttctatgt ggccggatac agcgcagtca ccgaggaggg
     6061 attggtagca gtgtccctgg ggtgccccaa actcaatagt ttgctgtact tctgtcatca
     6121 gatgaccaat gctgcccttg ttaccgttgc caaaaactgt ccaaacttca caaggtttag
     6181 actgtgcatc ttggagcccg gtaagcccga cgtcgtaacg tcacagccac tggacgaagg
     6241 gttcggtgcc atagtacgcg aatgtaaggg tctccagcgg ctgagcatat ccggcctgct
     6301 gactgacaaa gtgttcatgt atattgggaa gtacgccaag cagctggaaa tgctgtcaat
     6361 cgcattcgcc ggagattcag ataaagggat gatgcacgtg atgaacgggt gcaagaacct
     6421 gagaaaactc gaaattcgcg attccccttt tggggacgcc gcactgctgg gcaatttcgc
     6481 ccggtacgag acgatgaggt cactttggat gtcaagctgc aacgtgaccc tcaagggatg
     6541 ccaagtcctg gctagtaaaa tgccaatgct gaatgttgag gtcatcaacg aacgcgacgg
     6601 tagtaatgaa atggaggaaa accacggcga tctgcccaaa gtggagaaac tgtacgtcta
     6661 tcggaccaca gctggcgcac gagatgacgc tcccaatttc gtgaaaattc tgggcaagcc
     6721 catccccaac cccctgctgg gcctggacag caccggcagt ggagagggca gaggaagtct
     6781 gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg accgagtaca agcccacggt
     6841 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6901 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6961 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7021 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7081 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7141 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7201 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7261 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccacaacct
     7321 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7381 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagaa ttctaactag agctcgctga
     7441 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7501 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7561 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7621 ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgccctg caggatttaa
     7681 atgttaggtg ttatggcagt aactttaatt ctagcgatct ggaggcagag gcaggtggat
     7741 ctatgagttt gaggctggct tgctctacac agtgagttcc agaaaagcca gggctacaca
     7801 gtgagaccct atctagaaac aacaacagca acaaaaaata tacacatctc tcctaaaaat
     7861 aaaacacgca gtctggaagt aagtgaccgt tggggaagca ctggaaagga gttagagaat
     7921 gtagacctct tgtgcctgac tgggtgtccg gattcaatag tgacttctcc ggtgatctgg
     7981 gaatctgtga agaggttctg gctctctgtc tagcctgagg ctgatacccc aactcatttt
     8041 cttgtcttct gtgaggaagg ttacaactct aggagtcccc ttcctttggc cagtgcttcc
     8101 ctaagaaagc tatggcttga ttcccacccc caccctacct ccactgccaa gtctctggac
     8161 agatgtgcag cctctggcag tgggagggaa ggaactggat aaagtggtcc tcattcgtca
     8221 gcctcactgg caggcccgga aagtgctgaa ctcagggggc tccggagagg atgatgcaga
     8281 ggtgggcctc ttccgaggcc ctgagtctag ggttgccact gagtcttcct gagtcatttt
     8341 ctttgcccat ggaagaaaag aaaagaaatt taaaaaacaa aaacaaaaca aaaaacccta
     8401 agtggttttt ggtgtaagga tctgggagtt cctcctgttc gaagctgttg ggtaccttcc
     8461 tacctcttcc taccacctcg ccagatttca gattccgacc ttcagtggct caaatcccac
     8521 ttccctaact tcctagcagt ctgacctcac tttgagtgat aacacatatg agcctcagtt
     8581 tcaaatgcta gtatccatct cctaggggca gtatttaaat actggccgtc gttttacaac
     8641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8761 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     8821 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     8881 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8941 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     9001 caccgaaacg cgcga
//
