LOCUS       sequence-177217-       10643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse TIGRE acceptor locus using Puromycin selection. 
            Auxin-inducible degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN395 -
            pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-...
FEATURES             Location/Qualifiers
     source          1..10643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1351..1486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1591..1612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1627..1632,1633..1650,1651..1657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1665..1681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1689..1705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2064..2119
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2165..2184
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2275..2316)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(2317..4041)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(4100..4119)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4127..5135
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4181..4203)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(5136..5411)
                     /label=chicken beta-actin promoter
     enhancer        5414..5717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    5781..5814
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        5878..6377
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5913..5936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6310..6329
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6389..6436
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6455..7054
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6455..6474)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6951..6971
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7086..7103)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7092..7316
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7371..7404
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8422..8438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8446..8462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8470..8476,8477..8494,8495..8500))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8515..8536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8653..8670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8824..9412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8904..8923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9583..10374,10375..10443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10206..10225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10444..10548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10616..10634
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctaaccta ggcctccaaa aaagcctcct cactacttct
     1381 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     1441 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     1501 gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga
     1561 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1621 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1681 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg
     1741 atcttcataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
     1801 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
     1861 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
     1921 cagggggctg tttcatatac tgatgacctc tttatagcca cctttgttca tggcagccag
     1981 catatggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
     2041 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
     2101 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
     2161 taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc attggccaca
     2221 ccagccacca ccttctgata ggcagcctgc acctgaggcc gcttacccgg gtcaggtgct
     2281 gtccaggccc agcagggggt tggggatggg cttgcccaga attttcacga aattgggagc
     2341 gtcatctcgt gcgccagctg tggtccgata gacgtacagt ttctccactt tgggcagatc
     2401 gccgtggttt tcctccattt cattactacc gtcgcgttcg ttgatgacct caacattcag
     2461 cattggcatt ttactagcca ggacttggca tcccttgagg gtcacgttgc agcttgacat
     2521 ccaaagtgac ctcatcgtct cgtaccgggc gaaattgccc agcagtgcgg cgtccccaaa
     2581 aggggaatcg cgaatttcga gttttctcag gttcttgcac ccgttcatca cgtgcatcat
     2641 ccctttatct gaatctccgg cgaatgcgat tgacagcatt tccagctgct tggcgtactt
     2701 cccaatatac atgaacactt tgtcagtcag caggccggat atgctcagcc gctggagacc
     2761 cttacattcg cgtactatgg caccgaaccc ttcgtccagt ggctgtgacg ttacgacgtc
     2821 gggcttaccg ggctccaaga tgcacagtct aaaccttgtg aagtttggac agtttttggc
     2881 aacggtaaca agggcagcat tggtcatctg atgacagaag tacagcaaac tattgagttt
     2941 ggggcacccc agggacactg ctaccaatcc ctcctcggtg actgcgctgt atccggccac
     3001 atagaaatct gaagggaaca ccctcagctc ctgcagatct ttgcaggagg aggccacaac
     3061 ttgcagtccc ttgtctgaaa tacaatccag tacccagagc ctttggagct tcacgcagcg
     3121 gctgatcatc ttagtaaggt cactagcgtc gagggttgga gcgtaactca gattaagtcc
     3181 ggtcaactgt gcacacaggg gatatataaa agacaggcag acagggctag cgtcccaaaa
     3241 cccgctcagt gacctaagca ttttgcattt ttccagagca ctagtgagct taaagtagct
     3301 ctctgtctgg aaatcatcgg tcagatttcc ggtccccaga tcctccaggt tgggagtccg
     3361 gagcaaaatc ttggcgagcg tgtccacaga aacggatctg ttcagccgca gagatctcag
     3421 gttgggggat cgggaaacga gtctctccaa actcccggca ttcacttcgc ccttgataca
     3481 agcgaaattc aagctcacca ggctagtgca gctatccggg aaacagctca gccaccgagg
     3541 tcccctatcc tctacctcat tttcctgcag gtccagttct cgcagcagtt tacaatggct
     3601 agctacagca gccagaccgt cggtgctaaa tccttcacag ctgatcagca ccagggcgcg
     3661 gaatcggggg aagctgcggg caagcagttc cagtgactcg tctgacacca ccatcctttt
     3721 catcctgagt tcttccagac catgacaccc gcgcgcagcg gcctcgatcc agggcccggc
     3781 gtagccgccc cagtccggag gcaccaaatt gaagtcggca aagtgaggct tgcccttcac
     3841 tgtaagggcc ctgacgttcg ggaaccgggc ggccactcgc ccggctcgta cggcataaca
     3901 attacccaca aaaacacctc tgcggctcaa gcgttcgatc tcataccaga ccttgcacac
     3961 cagggacacg gtattcctat ctcgttgggc gggaaggaat gagaaaatat gttcgaccac
     4021 ctcctcaggg aagtatgtca tgatatcctg cagacgcgtg agaattctcg aggaattctt
     4081 tgccaaaatg atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga
     4141 agaaggcatg aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga
     4201 accccgccct gccccgtccc ccccgaaggc agccgtcccc ctgcggcagc cccgaggctg
     4261 gagatggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt
     4321 cctgccggcg ccgcaccgct tcgcccgcgc ccgctagagg gggtgcggcg gcgcctccca
     4381 gatttcggct ccgccagatt tgggacaaag gaagtccctg cgccctctcg cacgattacc
     4441 ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc gccggcgctc cggggccgcc
     4501 gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg gcacccccac
     4561 ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgcac ggagccccgc
     4621 acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg ggttacagcc
     4681 cgaccgccgc gcccacaccc cctgctcacc cccccacgca cacaccccgc acgcagcctt
     4741 tgttcccctc gcagcccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg
     4801 cacacgcgga gcgcacaaag ccccgcgccg cgcccgcagc gctcacagcc gccgggcagc
     4861 gcgggccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc
     4921 cccccgcaca aagggccctc ccggagccct ttaaggcttt cacgcagcca cagaaaagaa
     4981 acgagccgtc attaaaccaa gcgctaatta cagcccggag gagaagggcc gtcccgcccg
     5041 ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggcggcgcgg
     5101 agcggggcac ggggcgaagg caacgcagcg actcccgccc gccgcgcgct tcgcttttta
     5161 tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga
     5221 ttggctgccg ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg
     5281 cctggcgcgc gccccccccc cccccgcccc catcgctgca caaaataatt aaaaaataaa
     5341 taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc agaacgtggg
     5401 gctcacctcg acccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5461 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5521 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5581 gtaaatagtc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5641 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5701 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5761 attactatta ataactagtc gaagttccta ttctctagaa agtataggaa cttcaggtct
     5821 gaagaggagt ttacgtccag ccaagctagc ttggctgcag gtcgtcgaaa ttctaccggg
     5881 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5941 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     6001 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     6061 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     6121 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     6181 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     6241 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     6301 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     6361 catctccggg cctttcgacc tgcagcctgt tgacaattaa tcatcggcat agtatatcgg
     6421 catagtataa tacgacaagg tgaggaacta aaccatgacc gagtacaagc ccacggtgcg
     6481 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6541 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6601 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6661 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6721 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6781 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6841 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6901 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6961 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7021 cacctggtgc atgacccgca agcccggtgc ctgaggggat caattctcta gagctcgctg
     7081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7321 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaacccttc gaagttccta
     7381 ttctctagaa agtataggaa cttcggcgcg ccatttaaat tttgggatgt tttctgtttg
     7441 ttttgagata gggtttttca gtgtagtcct ggctgtccta gaactcactc tgtagaccag
     7501 gctggcctgg aactcaagga ctgccagcct ctgcctcctg agcatcggga ttaaaggtgt
     7561 gtgccaccac tgcccagcag agtgcgtttt tactcttcac tttgtgggtc ctggctgcct
     7621 gagattgtgt gcttgcacca ttagatggga gggatgactt gggaaagggt tgagtctttt
     7681 gtgagtcttg cggaggccca ggagggggta cgtaaattag agttctaaga ggcccagcaa
     7741 aagtgaacaa gtgactggcc actgtgagtt cactggggga cctgagagtt aactagtgaa
     7801 aaggagaggt cctagtggag aagctaaaga attaaagtta ctgccttgaa gttctttgtg
     7861 aaggccaagc tctggagcgc ggcttgaaaa gcctttatcc cagagcagca tgggaagggg
     7921 ccccagctgg gtgcgagcct gcaaaccaga gcacacctat cacagggcct tttcttacca
     7981 gtgggacggg atcaaggcgg agcttacctg ggaacgtaca gggcggaacc ttaacttggg
     8041 ggcgtggccg aatcacctgg gtttggaaac ggttcttgtc tttcctcaag ctcaggagag
     8101 gttgtttgca ggacactggc ccggaggggc ccgaactctc cgagggaggg tttcgtggat
     8161 cagaacaccg tcccttgaca aaaagatgca gagcaccgtc tgcattctgc tagtaaccgt
     8221 ccctcctggg ggcggagcca gccctcctcc gggtgtataa agtagctgag cgcctccttc
     8281 tgacaaacgc ctcaccccca agagtactgg gggagtacgc agcacgccag cctaggggcg
     8341 gagctcagcc ctggaggagg cgtttcttct tagttctccg actcacgcct ctccactttt
     8401 catttaaatg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8821 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8881 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8941 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9001 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9061 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9121 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9181 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9241 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9301 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9361 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9421 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9481 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9541 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9601 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9661 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9721 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9781 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9841 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9901 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9961 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10021 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10081 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10141 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10201 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10261 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10321 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10381 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10441 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10501 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10561 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10621 gcgtatcacg aggccctttc gtc
//
