LOCUS       sequence-177223-        8734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses V5-tagged human SYNJ2BP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
            Ting AY
  TITLE     Proteomic mapping of cytosol-facing outer mitochondrial and ER 
            membranes in living human cells by proximity biotinylation.
  JOURNAL   Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
  PUBMED    28441135
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-25"; volume: "6. pii: e24463. doi"; pages: " 
            10.7554/eLife.24463"
COMMENT     Sequence Label: pLX_TRC208-SYNJ2BP-V5
FEATURES             Location/Qualifiers
     source          1..8734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    complement(1034..1058)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1060..1101
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1120..1708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1173..1193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1591..1602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1780..2013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2091..2212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2128..2147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2182..2201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2252..2387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2407..2426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2408..2426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2436..2453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2436..2452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2445..2467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2594..3049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2681..2700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2891..2912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3075..3179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3180..3248,3249..4040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3398..3417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4211..4799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4700..4719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4953..4970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5087..5108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5123..5128,5129..5146,5147..5153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5161..5177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5166..5188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5185..5201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5185..5201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5220..5240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5222..5240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5268..5494
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5495..5675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5722..5847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6340..6573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6758..6802
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6977..7477
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7396..7414
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7489..8508
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    8565..8682
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atccaccatg
      601 aatggccgcg tcgattattt ggtcactgag gaggaaatta acctgacgcg cggcccctca
      661 gggctgggct tcaacatcgt cggtgggaca gatcagcagt atgtctccaa cgacagtggc
      721 atctacgtca gccgcatcaa agaaaatggg gctgcggccc tggatgggcg gctccaggag
      781 ggtgataaga tcctttcggt aaatggccaa gacctaaaga acctgctgca ccaggatgct
      841 gtagacctct ttcgtaatgc aggctatgct gtgtctctga gagtgcagca caggttacag
      901 gtgcagaatg gacctatagg acatcgaggt gaaggggacc caagtggtat tcccatattt
      961 atggtgctgg tgccagtgtt tgccctcacc atggtagcag cctgggcttt catgagatac
     1021 cggcaacaac tttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct
     1081 ctcctcggtc tcgattctac gtgaacgcgt taagtcgaca atcaacctct ggattacaaa
     1141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     1201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     1261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     1321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     1381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1501 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     1561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     1621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     1681 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     1741 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     1801 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     1861 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     1921 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     1981 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     2041 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2101 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2161 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     2221 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2341 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     2401 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     2461 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2521 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2581 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3001 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     3061 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3121 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3181 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3241 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3301 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3361 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3421 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3481 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3541 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3601 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3661 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3721 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3781 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3841 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3901 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3961 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4021 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4081 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4861 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4921 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4981 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5041 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5101 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5161 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     5221 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     5281 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     5341 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     5401 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     5461 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     5521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5641 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     5701 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     5761 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     5821 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     5881 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     5941 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     6001 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     6061 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     6121 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     6181 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     6241 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     6301 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     6361 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     6421 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     6481 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     6541 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     6601 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     6661 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     6721 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     6781 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     6841 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     6901 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     6961 aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     7021 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     7081 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     7141 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     7201 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     7261 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     7321 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     7381 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     7441 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccat gaaaaagcct
     7501 gaactcaccg ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     7561 ctgatgcagc tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt
     7621 ggatatgtcc tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat
     7681 cggcactttg catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc
     7741 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct
     7801 gaaaccgaac tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc
     7861 gccgatctta gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac
     7921 actacatggc gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact
     7981 gtgatggacg acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg
     8041 gccgaggact gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc
     8101 ctgaccgaca atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat
     8161 tcccaatacg aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag
     8221 cagacccgct acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct
     8281 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     8341 gatgcagctt gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc
     8401 gggcgtacac aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg
     8461 ctcgccgata gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa
     8521 tgagaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     8581 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8641 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8701 agcagagatc cactttggcg ccggctcgag gggg
//
