LOCUS       sequence-177236-       10646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Yi SJ, Lee HT, Groffen J, Heisterkamp N
  TITLE     Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for
            the small GTPase Rap1.
  JOURNAL   Int J Mol Med. 2008 Oct . 22(4):453-8.
  PUBMED    18813851
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2008 Oct . 22(4):453-8."
COMMENT     Sequence Label: pSG5-P190
FEATURES             Location/Qualifiers
     source          1..10646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(309..328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(310..328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(356..375)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          383..955
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(986..1182)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(994..1204)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1000..1135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1292..1312
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(1349..1366)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1349..1365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1358..1380)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1506..1961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1593..1612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1803..1824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2028..2047)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2147..2169
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2207..2225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2293..2397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2398..2466,2467..3258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2616..2635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3429..4017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3918..3937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4171..4188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    4222..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4233..4252)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4287..4306
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggcgcgacgc ggacgctcgg ggctcggagg cgccgtcggg ggcctgcgcc gcgcgccgga
       61 agccccatcg ctgccggtca tagctcttct tttccttggc cagcaacgtc tgcaggtaga
      121 tcatgcggaa gcgctcctgg ttcacctcct gctccaggcg ccgaatggag gccttgcagc
      181 gctccagctc ctgctcgatg tcgcccactg agcgcagctc catgcgcggg ggctctgagt
      241 ccgggaactg cgccttccac gcctccgcga agcccaccgg gtccaccatg gcgcggccat
      301 cgaattcgcc ctatagtgag tcgtattaca attctttgcc aaaatgatga gacagcacaa
      361 taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag
      421 aggggcccgg tttggactca gagtatttta tcctcatctc aaacagtgta tatcattgta
      481 accataaaga gaaaggcagg atgatgacca ggatgtagtt gtttctacca ataagaatat
      541 ttccacgcca gccagaattt atatgcagaa atattctacc ttatcattta attataacaa
      601 ttgttctcta aaactgtgct gaagtacaat ataatatacc ctgattgcct tgaaaaaaaa
      661 gtgattagag aaagtactta caatctgaca aataaacaaa agtgaattta aaaattcgtt
      721 acaaatgcaa gctaaagttt aacgaaaaag ttacagaaaa tgaaaagaaa ataagaggag
      781 acaatggttg tcaacagagt agaaagtgaa agaaacaaaa ttatcatgag ggtccatggt
      841 gatacaaggg acatcttccc attctaaaca acaccctgaa aactttgccc cctccatata
      901 acatgaattt tacaatagcg aaaaagaaag aacaatcaag ggtccccaaa ctcaccctga
      961 agttctcagg atcgatccgg agctttttgc aaaagcctag gcctccaaaa aagcctcttc
     1021 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     1081 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     1141 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     1201 ctgctgggga gcctggggac tttccttgct gactaattga gatgcatgct ttgcatactt
     1261 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
     1321 ttctttccgc ctcagaagtc gacaattcac tggccgtcgt tttacaacgt cgtgactggg
     1381 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1441 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1501 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1921 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     1981 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2041 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4201 tctagagtcg accagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4261 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4321 ttataagctg caataaacaa gttctgcttt aataagatct ggatccccgg gaattctaat
     4381 gtaaacactg atttatttaa aaaatactac agaggacaga tttggattca aaaaaaacca
     4441 cagagaatca tatacagaaa caaaatgcac tggaccccga gaagcagagg gggagccacc
     4501 acaggccccc gagggggcag ggcaggacgc tatgcacacg ccacttagaa aagagcgtct
     4561 ataaaagatg cataccaaga gatacgtgta aaatatagtg gcacatcacg ttaacaaaag
     4621 gaagggacca gtacctcatg gagacaggca gtcgggggac ctgggtgggc gctttgctct
     4681 gtcttcaagg ccgctgtcgg tgactagtgc ctccctccgg ctgcgtcaca gccgtcgggg
     4741 gcaaagaggt gatgtgctgt aagaaccgca taaaacgatc cagaggaaac catttctatc
     4801 agaaaaataa agtacaagtt taggtgatga gcgtaaagct agggcctgtg cgaggctgct
     4861 ggcacgaggc ggtatgaagg ggtgggaagg tggcttctcc cggcgtgaag cctcaggggc
     4921 ggccacctgc ccactgctct cggagttagc tgctctgtca aggtccagga gaccccgggt
     4981 ccctgcccag agtcacagcc cccgtatctg ggctccggcc tggggcagcc tcggggagaa
     5041 gatggcaggc gtgctctgtg aaatacgtga ggctttcagg ggctccgttt gtggttttcc
     5101 taaccctccc tgatgacccc cacagaacat gaggctatgt cactcacggt aattaaataa
     5161 gacattcgcc cagttcaaga gtgcagccag gaccaggact ggccaggccc tttcctccca
     5221 cctgtgcgca ggtgcccagt ggcagctcta ttctctctca gggcggctac acagaggcag
     5281 ggctttgaaa tgcttcaaga actcagaaaa gtgaaggctg tcttgttcca aaggaaaggg
     5341 ccttgtaaga atctactttg tcttagagga gtgggggagg ggaagggccc tttcagggca
     5401 cccagatgtg tgaaaggacg gttcactctg ggcccttgca gacctgcctg ggcccagtct
     5461 ggggctattt ggcaccagct gttcacccca ctagccactg cctcccttgt cccctgcctc
     5521 cattctgctg ggggagggat gtcggcagtg acagtgacct gccacccttg agactcaagc
     5581 tttcttgcca ggattcacag tgcacgtcac aagagagcaa gagctgggct ctggggatgt
     5641 ggacagatgg aaaggacatg ccccatgcct cgatcctttt cagctcccac ctgcccagga
     5701 cccaaaggct ggttggcttt agcaccctgt tttccaccca aatcaagagc tgtcctgcac
     5761 gtgaggaaat ggcgagtgca ccccaccccg ggcagcagca gagggcagag tgaccagccg
     5821 tgtgcatgcc cgtgcatgcc cttcccgaaa tgcggttctt gaaacatgtc ttcagcacac
     5881 gacgggcaag gccagggagt gcaggcagag ggcctgacac acaaggcctc agttcaggct
     5941 tccattcttg gagaagtgga agaaggaggc ccagccgggg gaggaggggc tccagagaga
     6001 aaaaaatgaa aaagacagtc cacatcagcc gttcccacct ggcctggaga gctcagtctg
     6061 gggcactagg tgggagcggg gctggcgggt cctggcatgc cgactgcagt ccacggagca
     6121 ggaactccac agataaaatt cgaggacaga gacggagcgg ggaggaggaa ggtgcgggag
     6181 ggcaggcggt gcaaacgtag gtagtagagc tggactccac agggccctca gctctgcctg
     6241 gcgcagagct cctgggccaa ggtgctgact cactagtccc ttgtcagcca ctgtcacggg
     6301 tatgggcgag cccgcatgtg ctgcaggcag ctccgacggg cctgacacct gacccctgac
     6361 tgctgctacc tctgcactat gtcactgatt tccttcaccg aactgaggag cttgctgaag
     6421 tcctgagtgg ccgctggacc actgcctgct gtcgccgggc agatctgaag ctcccggaga
     6481 ttattctcca gtttgttgat ggcctctcgg aaggcaaact tgttcctcat ttgctggatg
     6541 gaatccacat agctcacgca gaacgtgtag aggtttttgc cggcctccag cactgcgctg
     6601 tggctggcca tctgctcgga gttcctagag atggcgaggc acagcgcctc ggtgctgtcc
     6661 aggaccacgc ccttggtgat ggcgccgctg gcgatccgct ctggaggctg gcgggttttc
     6721 cgaagagaca ctcgggttga tatgagaggg atgaaggcgg tggaagacgg ctggtcccct
     6781 gccagggccg aggatgctga tggcaacgtg gagggaacgg gggctgggct gatgggggtc
     6841 cccgacggct tggcggactg tggctttgga gtggccggga gcacgggctt tttgaggccc
     6901 tctcccggct ggctgggctt ggcagcgtca ctgttcacag catcaaccag actcgtggct
     6961 tttgtctttg cgcccaggac tgcctccccg gccgcctcct ggctcgggct ctgcgagggc
     7021 tttcctccag ccttccctgc agaggctgct ggtgggggcg gcggggcagg tttgagcctg
     7081 gacaatttcc ccttgtccct ccctggcgac tcagaggagt gcttgtgcct cctcactctg
     7141 gactcctcgg cggggccctt gctggtgccc cctggtgcac ctgagcctgc tttgctggtg
     7201 ggggtcactg gctcagctgc agcaggggtc cctaaggcac tgccctttcc agcttcttcc
     7261 ttgtggggga ggcccgaggc aggggccacg gtgacctgcc gccggagggg ttttggagtc
     7321 aggttgggcg ggctggagcc cgggctggac tccatgatgt ctttgaagac ctcatcagca
     7381 gcttcctcat tctttttcac cagcctgggg ggaggcgtta ctgtgcctcg ggtcacctgg
     7441 tcagacctgt tctcccctgc cctcttccga ggcagagccg gcttctcact tttgtgccct
     7501 ccaaatgtgg acgagtcaaa ctgtcttccc gtggactgca agtcccgagg cagcgtgact
     7561 gacctccact ccgtgtcctt ggccccatgg ggaacgcagg aggcggagca agagcgcagg
     7621 aagcgcttgc tggagctgcc accgccctcc tcctcgccgg tggctaggcg gctgctggtc
     7681 agcgtgctgg acttcttcca caggtgggga gaccggaagc ctgagccccc ggactcccgg
     7741 agggctccat tggggacccc agccccattg ctgggctttg gggacttggc tgggtcagct
     7801 gtgtccaagg gggtgaaagc cagtgccccg ttgctgatgt ctcggccctc ttcctcgccg
     7861 gcccctctgc gctccggctg gccgtccatc tcccggaagg agctgctgcg tttgggaggg
     7921 gttggggctg tcttcttctt cttcttgatc aaggcgctga acaagttggt ctttttgtct
     7981 ttggggagaa ggcgctcatc ttcattcagg ccgccctccg ggggacctcg ctcttttcga
     8041 gggagcaatg gagacacggc aggctcatgg tccagaggat cgctctctcc ctggcccttg
     8101 gagtgaggca tctcaggcac gtcagtggtg tctctgtgct ctgcagctct cctggaggtc
     8161 ctcgtcttgg tgggcagctc tggggcctgc agcaaggtac tcacagcccc acggacgcct
     8221 tgtttcccca gctccttttc cacttcgtct gagatactgg attcctggaa cattgtttca
     8281 aaggcttggt ggatttcagc aaaggagggc cggtcagagg gattccactg ccaacatgct
     8341 cgcatgagtt catagacctt ctctgggcag ccttctgggc gctccatgcg gtagtccttc
     8401 tctagcagct catacacctg ggacaggtca attcccgggt aaggggacat gccataggta
     8461 gcaatttccc aaagcaatac tccaaatgcc cagacgtcgg acttgatgga gaacttgttg
     8521 taggccaggc tctcgggtgc agtccatttg atggggaact tggctccagc atgggctgtg
     8581 taggtgtccc ctgtcatcaa cctgctcagg ccaaaatcag ctaccttcac caagtggttc
     8641 tcccctacca ggcagtttcg ggcagcaaga tctctgtgga tgaagttttt cttctccagg
     8701 tactccatgg ctgacgagat ctgagtggcc atgtacagca gcaccacggc gttcacctcc
     8761 tgccggttgc actccctcag gtagtccagg aggttcccgt aggtcatgaa ctcagtgatg
     8821 atatagaacg ggggctcccg ggtgcagacc ccaaggagct gcaccaggtt agggtgtttg
     8881 atctctttca tgactgcagc ttctttcaag aactcttcca cctccatggt gtcctccttc
     8941 aaggtcttca cggccaccgt caggctgtat ttcttccaca cgccctcgta cacctccccg
     9001 tactggcccc cgcccagctt gtgcttcatg gtgatgtccg tgcgttccat ctcccacttg
     9061 tcgtagttgg gggacacacc atagacagtg ggcttgttgc gctttggggc tggataatgg
     9121 agcgtggtga tgagcccgtc ggccaccgtt gaatgatgat gaaccaactc ggccagggtg
     9181 ttgaagcggc tctcggagga gacgtagagc ttgccatcag aagcagtgtt gatcctgtaa
     9241 tggtacaccc tcccttcgta tctcagcgag atggacctct ggccaggact gctctcactc
     9301 tcacgcacca agaagctgcc attgatcccg ctgctcagca gatactcagc ggcattgcgg
     9361 gacacaggcc catggtacca ggagtgtttc tccagactgt tgactggcat gatgtagttg
     9421 cttgggaccc agccttggcc atttttggtt tgggcttcac accattcccc attgtgatta
     9481 tagcctaaga cccggagctt ttcaccttta gttatgctta gagtgttatc tccactggcc
     9541 acaaaatcat acagtgcaac gaaaaggttg gggtcatttt cactgggtcc agcgagaagg
     9601 ttttccttgg agttccaacg agcggcttca ctcagaccct gaggctcaaa gtcagatgct
     9661 actggccgct gaagggcttc tgcgtctcca tggaaggcgc cctcgccatc gttgggccag
     9721 atctgcccgg tcttgcggac gcccacgatg gtggcctcgg acacgacaac cgggcagtgc
     9781 cggtgccgct tatggcactg cggcgtgggg ggactgctgc tgtcgaagga ctgttgcgag
     9841 ttctgcgagg gagagcggct tttgtcccgg aacatgcggt aggtggtggg gcttggggac
     9901 acgcggctgg actggccaga ggagaagtcc tcctcgctgg aggtgaggtt ctcattggag
     9961 ctgcagtccg gggtatagcc gcctccgcaa tcctcaaaac tccgggggga gtaggacctg
    10021 cggggccagg taaggcgctt ctcctgctca gaggtgctct ggctgcgcag gagcgggccc
    10081 ttgccctccc cttccatcat gcccccgacg tagatgctct ggtagggctg gtactccagg
    10141 ggcggccaag ggggcctgct accgccattg gcgtcgatca ggttgtcctt caggaagcgg
    10201 gggttcaact cggcgtcctc gtagtcgccg tcgacgccgc agctgctctc cgaggagcgt
    10261 ccccggtaag ggggcctgga tgcatccccc acgctcgagc ccgcgccgtg ctgggacttt
    10321 ttgcgctcca tctgcatggc ctggctgccc agggagctga tgcggtccga cacctctttg
    10381 tcgttgacct tcaccaggcc gcgctcgtgg tgaaactcga cgttcacgta gaagggcttc
    10441 tcggcgtccg ccccgggctg gccatggccc ttgcgtatcc gctcgaagtt ggacctgagc
    10501 gccgccacgc tggcgggggg tccccggtcg tcccgttccc ccgacgcggc tgccccgggc
    10561 ctgcgggcgg tcccgggcct ggccttaccc ggagaaccct cgccgtcggg ccgggcctcg
    10621 ggctcctcgg cgggcggcgg gtcggc
//
