LOCUS       sequence-177253-        8798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR
  TITLE     Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and 
            IRS-2-mediated signaling.
  JOURNAL   Mol Cell Biol. 2001 Jan . 21(1):26-38.
  PUBMED    11113178
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 Jan . 21(1):26-38."
COMMENT     Sequence Label: pBABE puro mouse IRS-1 myc
FEATURES             Location/Qualifiers
     source          1..8798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(174..191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(345..933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(425..444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1093..1884,1885..1953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1716..1735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1954..2058)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             2089..2558
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2623..2822
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3023..3439
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3369..3391
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3407..3423
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             7165..7194
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7209..7229)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7214..7543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7394..7529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7553..8152
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7553..7572)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8049..8069
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             join(8373..8798,1..44)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
       61 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
      121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1021 aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat atgagtaaac
     1081 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1141 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1201 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1261 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1321 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1381 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1441 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1501 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1561 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1621 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1681 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1741 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1801 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1861 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1921 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1981 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2041 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcagcct gaatatgggc
     2101 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
     2161 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     2221 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     2281 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     2341 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     2401 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
     2461 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     2521 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
     2581 ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc cagcaactta
     2641 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta
     2701 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
     2761 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
     2821 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
     2881 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
     2941 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     3001 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     3061 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     3121 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     3181 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     3241 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     3301 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     3361 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     3421 cactccttct ctaggcgccg gccggatccg catgcgagct cgagcatggc gagccctccg
     3481 gataccgatg gcttctcaga cgtgcgcaag gtgggctacc tgcgcaagcc caagagtatg
     3541 cataagcgct ttttcgtgct gcgggcggcc agcgaggccg ggggcccagc gcgcctggag
     3601 tattatgaga acgagaagaa gtggcggcac aagtcgagcg cccccaaacg ctcgatcccc
     3661 ctcgagagct gtttcaacat caacaagcgg gctgactcca agaacaagca cctggtggct
     3721 ctctacaccc gagacgaaca ctttgccatt gccgcggata gcgaggctga gcaagacagc
     3781 tggtaccagg ctcttctgca gctgcataat cgggcaaagg cccaccatga cggggctgga
     3841 ggaggctgcg gcggtagctg cagcggcagc tctggcgttg gagaggcagg ggaggacttg
     3901 agctatgaca cgggtccagg acccgcgttc aaggaggtct ggcaggttat cctgaaaccc
     3961 aagggcctgg gtcagacaaa gaacctgatt ggcatctacc gcctctgcct gaccagcaag
     4021 accatcagct ttgtgaagct gaactccgag gcagccgctg tggtgctgca gctgatgaat
     4081 atcagacgct gtggccactc agagaacttc ttcttcatcg aggtggggcg ttccgctgtg
     4141 acagggcctg gcgagttctg gatgcaagtg gatgactccg tggtggccca gaacatgcat
     4201 gagaccattc tagaggcaat gagggccatg agcgatgagt ttcgccctcg cagcaaaagc
     4261 cagtcttcat ccagttgctc caaccccatc agcgttcccc tgcgcaggca ccatctcaac
     4321 aaccctccac ccagccaggt gggactgact cggagatctc gaactgagag catcactgcc
     4381 acctcccctg ccagtatggt gggtgggaaa ccaggttcct tccgggtgcg tgcctccagc
     4441 gatggcgaag gcaccatgtc ccgtccagca tcagtggatg gcagtcctgt gagccctagc
     4501 accaacagga cccacgccca tcggcatcga ggcagctcca ggctgcaccc cccactcaac
     4561 cacagccgct ccatccccat gccttcttct agatgctcac cttcagccac cagcccagtg
     4621 agtctgtcat ctagtagtac cagtggccat ggctccactt cagactgtct cttcccgagg
     4681 cgctctagtg cttccgtgtc cggttcgcct agcgatggcg gtttcatctc ttctgatgag
     4741 tatggttcta gtccctgcga tttccgaagt tccttccgca gtgtcacccc agattccctg
     4801 gggcacaccc caccagccag gggtgaggag gagctgagca attatatctg catgggtggc
     4861 aagggagcct ccaccttggc tgctcccaat ggccactaca ttttgtctag gggtggcaac
     4921 ggccatcgct acatcccagg tgctaacttg gggacaagcc cagcgctgcc tggagatgaa
     4981 gccgcgggtg cagcagatct ggataaccgg tttcgaaaga gaactcactc cgcaggcaca
     5041 tctcctacca tttcccatca gaagaccccc tcacagtctt cagtggcttc tattgaggaa
     5101 tatacagaga tgatgcccgc tgcctaccca ccaggaggtg gcagtggagg ccgactgccc
     5161 ggctaccggc attccgcctt cgtgcccacc cactcctatc ccgaagaggg tctagagatg
     5221 caccacttgg aacgtcgtgg aggccaccac cgtccagaca cctccaacct ccacactgat
     5281 gatggctata tgcccatgtc tcctggggtg gctccagtgc ccagcaaccg caaaggaaat
     5341 ggggactata tgcccatgag ccccaagagt gtatctgccc cacagcagat cattaacccc
     5401 atcagacgcc acccacagag agtggacccc aatggctaca tgatgatgtc acccagtggt
     5461 agttgctccc ctgacattgg aggtgggtcc agcagcagta gcagcatcag cgcagcccct
     5521 tctgggagca gctatgggaa gccatggaca aatggagtag gggggcacca tactcatgcc
     5581 cttcctcatg ccaaacctcc tgttgagagt ggtggcggta agctcttgcc ttgcacaggt
     5641 gactacatga acatgtcccc agtgggagat tccaacacca gcagcccctc agaatgctac
     5701 tatggcccag aagatcccca gcacaagccg gtcctctctt actactcatt gccaaggtcc
     5761 tttaagcaca cccagcgccc tggagagcca gaggagggtg ccaggcacca gcatcttcgt
     5821 ctctcttcta gctctggacg ccttcgctat accgcaactg ccgaagattc ctcctcttct
     5881 accagcagcg acagtctggg tgggggttac tgtggggcta ggccagagtc aagcctcaca
     5941 catccccacc accatgtctt gcagccccac ctgcctcgaa aggtagacac agctgcacag
     6001 accaacagcc gcctggctcg acccacaagg ctgtccttgg gggatcccaa ggcaagcacc
     6061 ttaccccggg ttcgagagca gcaacagcag cagcagtctt ccctgcaccc tcccgagccc
     6121 aaaagcccag gagaatatgt gaatattgaa ttcgggagcg gccagcctgg ctatttagct
     6181 ggccctgcaa cttcccgtag ctctccttca gttcgatgtc caccccagct ccacccagct
     6241 cctagagaag agactggctc ggaagagtac atgaacatgg acttggggcc aggccggagg
     6301 gcaacctggc aggagagtgg tggagttgag ttgggcagaa taggccctgc acctccgggg
     6361 tctgctacgg tttgcaggcc aacccgttcg gtgccaaata gccgtggtga ctacatgacc
     6421 atgcagatag gttgtcctcg tcaaagctat gtggatacct caccagtggc cccagtcagc
     6481 tatgctgaca tgcggacagg cattgctgca gagaaggcga gcctgcctag acccacagga
     6541 gctgctcctc ctccatcctc cacagcctct tcttctgtta cacctcaagg agccaccgct
     6601 gagcaagcta ctcactcttc cttgctggga ggccctcagg gacctggggg catgagtgca
     6661 ttcaccaggg tgaacctcag tcccaaccat aaccagagtg ccaaagtgat tcgcgcagac
     6721 actcaagggt gccggaggag gcatagctct gagaccttct cagcacctac tcgggctggc
     6781 aatacggtgc cctttggagc aggggctgca gtagggggca gcggcggtgg tggtggtggc
     6841 ggcagtgagg atgtaaaacg ccacagctct gcatcctttg agaatgtgtg gctgagacct
     6901 ggggatctag ggggagtctc caaggagtcg gctccagtgt gtggggctgc tgggggtttg
     6961 gagaagagtc ttaactacat agacctggat ttggccaagg agcgctctca ggactgcccc
     7021 tctcaacagc agtccctacc accccctccc cctcaccagc ccttaggcag caatgagggc
     7081 aactccccaa gacgctccag tgaggattta agcaactatg ccagcatcag cttccagaag
     7141 cagccagagg atcgtcaagt taacgagcaa aagctaatta gcgaggagga cctttaggtc
     7201 gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7261 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7321 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7381 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7441 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7501 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga
     7561 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7621 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     7681 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7741 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7801 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7861 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7921 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7981 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8041 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8101 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     8161 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga taaaataaaa
     8221 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     8281 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa
     8341 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     8401 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     8461 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     8521 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     8581 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     8641 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     8701 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     8761 agttgcatcc gacttgtggt ctcgctgttc cttgggag
//
