LOCUS       sequence-17728-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and 
            siRNAs.
  JOURNAL   Mol Cell. 2004 Jul 23. 15(2):185-97.
  PUBMED    15260970
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 23. 15(2):185-97."
COMMENT     Sequence Label: pIreSneo-FLAG/HA Ago3
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             47..73
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcgatatcg ccggccgcca tggactacaa ggacgacgat gacaagtacc cttatgacgt
       61 gcccgattac gctagcggcc gcatggaaat cggctccgca ggacccgctg gggcccagcc
      121 cctactcatg gtgcccagaa gacctggcta tggcaccatg ggcaaaccca ttaaactgct
      181 ggctaactgt tttcaagttg aaatcccaaa gattgatgtc tacctctatg aggtagatat
      241 taaaccagac aagtgtccta ggagagtgaa cagggaggtg gttgactcaa tggttcagca
      301 ttttaaagta actatatttg gagaccgtag accagtttat gatggaaaaa gaagtcttta
      361 caccgccaat ccacttcctg tggcaactac aggggtagat ttagacgtta ctttacctgg
      421 ggaaggtgga aaagatcgac ctttcaaggt gtcaatcaaa tttgtctctc gggtgagttg
      481 gcacctactg catgaagtac tgacaggacg gaccttgcct gagccactgg aattagacaa
      541 gccaatcagc actaaccctg tccatgccgt tgatgtggtg ctacgacatc tgccctccat
      601 gaaatacaca cctgtggggc gttcattttt ctccgctcca gaaggatatg accaccctct
      661 gggagggggc agggaagtgt ggtttggatt ccatcagtct gttcggcctg ccatgtggaa
      721 aatgatgctt aatatcgatg tttctgccac tgccttctac aaagcacaac ctgtaattca
      781 gttcatgtgt gaagttcttg atattcataa tattgatgag caaccaagac ctctgactga
      841 ttctcatcgg gtaaaattca ccaaagagat aaaaggtttg aaggttgaag tga
//
