LOCUS       sequence-177290-        8002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Caruso JP, Koch BJ, Benson PD, Varughese E, Monterey MD, Lee AE, 
            Dave AM, Kiousis S, Sloan AE, Mathupala SP
  TITLE     pH, Lactate, and Hypoxia: Reciprocity in Regulating High-Affinity 
            Monocarboxylate Transporter Expression in Glioblastoma.
  JOURNAL   Neoplasia. 2017 Jan 13;19(2):121-134. doi: 
            10.1016/j.neo.2016.12.011.
  PUBMED    28092823
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neo.2016.12"; journalName: "Neoplasia"; date: 
            "2017-01-13- 13"; volume: "19"; issue: "2"; pages: "121-134"
COMMENT     Sequence Label: pGL2-MCT2Prom2.5
FEATURES             Location/Qualifiers
     source          1..8002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2479..4131
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2545..2563)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4372..4437
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4567..4587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5012..5146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5288..5305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5459..6047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5539..5558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6218..7009,7010..7078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6841..6860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7079..7183)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7210..7665
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7297..7316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7507..7528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7855..7976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ccgggagaat ccttaaaatg ctatagaagt gatgatatat ttgctgggca ttaaaggagg
       61 aaaaaaggtt tagacaagtt tgatatggaa aagagggcag tgaatgagaa aagacagaaa
      121 aatgtgagaa gattgtgtat gactgaggaa tgaagaaggg agaaagaacc taaaccacaa
      181 ggatgtgggt atatagtaaa agacatgtgg acttcatgag tatatatgtg atgtacgtat
      241 tcacagtttg ctcatctgtg gaatatgagt tgctagaatg ctaagtgtat tgctatttgt
      301 ttttcccact gttatttcag cttatgaata taaatcaagg caaacatttt accagagcat
      361 tccaagttct aatcatatgg cttatttttt ccctaaattg tatttttttc tcaatttctt
      421 aacaatatta aactgtaagt cataggataa actggctgaa tttattctta gctgattctc
      481 cctcctttca gatacctttc tagtccaatc aaattttaca taacatacct agctatcaca
      541 acaaagcaat ttagaaatga gttcccaaat actaactctg tgacagtagg taaattagat
      601 ccagagtttt aattgttgaa aaaacaagac ttaaaaagaa ggttacgata tcatgctata
      661 agtagtgatg tttggcattc agcaataggt taaacaggtc aagaacatgg gactataaga
      721 aaaaaacaac aaaaaagaaa aatcaaagag aaggaaaagg gagaatagga tcaaaattca
      781 gaaagcatat ggtctttctt cttcctcttc tttgacagga aataaaagtg atgaatgagc
      841 aggaaaaata tcaatagcaa acactcaaga ctatgacctt gaaatgtgcc tttatgttgt
      901 ttcttttaaa tgtttaactt tttttaatta aatgaagtgt ttatagtcac tgctagacat
      961 ttcatcatta tcaaaggcct attatgtctt atacaaatgt ctatttaagc atttttagct
     1021 taaaaatata aaatcctttc tgtaattttc tgcctttttt tcattttcaa atatcattct
     1081 ttcaataaaa ttatcttcca gtaattttga tgttattaat cacgattatt ttttcatcgt
     1141 gtattatgcc taccagccac ttcatgtata gtatttcact taaatcttat gaaatcaaca
     1201 cgattgatat tacttttatt tcatagctac agaaattgaa attcaaattt cgtccattac
     1261 aacagactag ctgtggcaaa gacaatgtga atttacccaa atgacttaac tctttttata
     1321 aattcgtaaa atgtgaagaa tcatagaaca caccttaaaa tgattgtgaa atcaaggagg
     1381 agaaagctag ctaaactggt agacaaactg caataggact tgtcaagcca aaacctacaa
     1441 ttattctttt ggatagtttc tttttctcta aagtggcaat ttaacttaaa gatattaaag
     1501 aaacccaatc tatggggtta cttaaatgtt ctcttccagc ccttcctctt tccagtttta
     1561 ccaagaagca cattttagac aggggaagag accaaaccaa tgacagcaga attaccatta
     1621 tctctagcac tattcgttgt caaataacta aatgctggtt tgaagaacta agtaatttga
     1681 gaaattaaaa atgaaaggaa ttataaatga tttgaaaaaa atccttctta tttttaatgt
     1741 tttttggtgc tgttttctat gtccttcctc ctatggtgtc aattatattt ttttccgtga
     1801 agtgatttgg ttggtgagtc taatctccaa cagtgcccta cctctttctc ccaaaaaggc
     1861 ctcagttgaa aaatggctca acactttccc tgcaagctca caatgaggat ctcctattct
     1921 ccctgcattc ggccagactc ttcttttttt gcatgctttc ctctcccatg cttgggttgc
     1981 acagagctac cgcacatctg tcttctaggt caggctacag agtacttcgg atacagcaag
     2041 ttacgaccca tctggaaaga cagatctacc tgccttttgg ctttcttcct tggtcaaaac
     2101 taaaatccga agtggagtgc cccgagcgat agagaaagtg cctatagctt tgacagccgc
     2161 ttggagcatg ctcagtcggt ctccaggcac cgggaggcgg ctcccccccc accacccccc
     2221 gggccggcct ccaccgcagc ctgtccggcc cccacccctg gcgcgcgctc ctccgcaccc
     2281 acaagggcgt tcagcagccg cgcctcccct tcccgccacc gcctccttct ccgctggctg
     2341 tggcgggcga ggacacgtca ggggccataa ataaataaat aattccattc acccgttgac
     2401 agcgaggcga atcggctgcg gtttgctggg aacggagggc tgcagcgcca agcttggcat
     2461 tccggtactg ttggtaaaat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     2521 tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     2581 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg
     2641 gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     2701 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     2761 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     2821 ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa
     2881 aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct
     2941 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     3001 ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg
     3061 ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact
     3121 gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     3181 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     3241 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta
     3301 cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc
     3361 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     3421 gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca
     3481 gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     3541 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     3601 gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga
     3661 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     3721 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata
     3781 gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg
     3841 gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac
     3901 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     3961 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     4021 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     4081 atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta aaatgtaact
     4141 gtattcagcg atgacgaaat tcttagctat tgtaatactg cgatgagtgg cagggcgggg
     4201 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa
     4261 gtgataataa gcggatgaat ggcagaaatt cgccggatct ttgtgaagga accttacttc
     4321 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     4381 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     4441 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     4501 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     4561 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     4621 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     4681 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     4741 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     4801 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     4861 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     4921 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4981 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     5041 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5101 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgtc gaccgatgcc
     5161 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     5221 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     5281 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5461 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5881 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6181 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6241 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6301 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6361 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6421 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6481 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6541 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6601 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6661 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6721 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6781 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6841 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6901 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6961 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7021 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7081 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7141 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7201 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7261 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7501 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7561 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7621 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca
     7681 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
     7741 agctaccatg ataagtaagt aatattaagg tacgtggagg ttttacttgc tttaaaaaac
     7801 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7861 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7921 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatggt
     7981 actgtaactg agctaacata ac
//
