LOCUS       sequence-177319-        6059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Flag-MCPIP1(1-453).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M
  TITLE     MCP-induced protein 1 deubiquitinates TRAF proteins and negatively 
            regulates JNK and NF-kappaB signaling.
  JOURNAL   J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. 
            Epub 2010 Nov 29.
  PUBMED    21115689
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20092641"; journalName: "J Exp Med"; date: "2010-12-20-
            20"; volume: "207"; issue: "13"; pages: "2959-73"
COMMENT     Sequence Label: Flag-MCPIP1(1-453)
FEATURES             Location/Qualifiers
     source          1..6059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             645..668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2083..2705
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2282..2301)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2729..2749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2734..3063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2914..3049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3101..3120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3102..3120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3134..3150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3134..3150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3147..3169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3158..3174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3182..3188,3189..3206,3207..3212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3227..3248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3365..3382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3536..4124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3616..3635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4295..5086,5087..5155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4918..4937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5156..5260)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5287..5742
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5374..5393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5584..5605
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5868..5890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5882..5899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5883..5899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggcc cacaaccacc gccacaagca cgccgactac aaagacgatg
      661 acgacaagct tgcggccgcg aattcaatga gtggcccctg tggagagaag cctgtcctgg
      721 aagccagccc caccatgagt ctgtgggaat ttgaggacag ccacagccgt cagggcaccc
      781 caaggccggg tcaagagctg gccgctgagg aggcctcggc cctggaactg cagatgaagg
      841 tggacttctt ccggaagctg ggctattcat ccacggagat ccacagcgtc ctgcagaagc
      901 tgggcgtcca ggcagacacc aacacggtgc tgggtgagct ggtgaaacac gggacagcca
      961 ccgagcggga gcgccagacc tcaccggacc cctgccctca gctccctcta gtcccgcggg
     1021 gtggtggcac ccctaaggct cccaacctgg agcctccact cccagaagag gaaaaggagg
     1081 gcagcgacct gagaccagtg gtcatcgatg ggagcaacgt ggccatgagc catgggaaca
     1141 aggaggtctt ctcctgccgg ggcatcctgc tggcagtgaa ctggtttctg gagcggggcc
     1201 acacagacat cacagtgttt gtgccatcct ggaggaagga gcagcctcgg cccgacgtgc
     1261 ccatcacaga ccagcacatc ctgcgggaac tggagaagaa gaagatcctg gtgttcacac
     1321 catcacgacg cgtgggtggc aagcgggtgg tgtgctatga cgacagattc attgtgaagc
     1381 tggcctacga gtctgacggg atcgtggttt ccaacgacac ataccgtgac ctccaaggcg
     1441 agcggcagga gtggaagcgc ttcatcgagg agcggctgct catgtactcc ttcgtcaatg
     1501 acaagtttat gccccctgat gacccactgg gccggcacgg gcccagcctg gacaacttcc
     1561 tgcgtaagaa gccactcact ttggagcaca ggaagcagcc gtgtccctat ggaaggaaat
     1621 gcacctatgg gatcaagtgc cgattcttcc acccagagcg gccaagctgc ccccagcgct
     1681 ctgtggcaga tgagctccgt gccaatgctc tcctctcacc ccccagagcc ccaagcaagg
     1741 acaaaaatgg ccggcggcct tcaccttcat cccagtccag ctctctgcta acagagagtg
     1801 agcagtgcag cctggatggg aagaagctgg gggcccaggc atccccaggg tcccgccaag
     1861 agggtctaac acagacctat gccccatcag gcaggagcct cgcacctagc gggggcagtg
     1921 gcagcagctt tgggcccaca gactggctcc cacagacgct ggactcactc ccgtacgtct
     1981 cccaggattg cctggactcg ggcattggct ccctggagag ccagatgtcg gaactttggg
     2041 gggtttaatc tagatatcgg taccggatcc agatctgatc ccgggtggca tccctgtgac
     2101 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2161 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     2221 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     2281 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     2341 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     2401 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     2461 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     2521 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     2581 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     2641 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     2701 gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2761 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2821 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3001 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3061 aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt ccctatagtg agtcgtatta
     3121 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5281 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5461 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5521 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5581 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5641 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5701 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
     5761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     5821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5881 ttgtaaaacg acggccagtg ccaagctgat ctatacattg aatcaatatt ggcaattagc
     5941 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     6001 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgtt
//
