LOCUS       sequence-177327-        2215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in 
            with BbsI, out with BsaI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG
  TITLE     An efficient tool for metabolic pathway construction and gene 
            integration for Aspergillus niger.
  JOURNAL   Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004.
  PUBMED    28533066
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour 
            Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 
            10.1016/j.biortech.2017.05.004."
COMMENT     Sequence Label: BB2_L_BC_syn_BbsI
FEATURES             Location/Qualifiers
     source          1..2215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     279..296
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        561..665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(666..734,735..1526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(884..903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(1752..2215,1..125)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
       61 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      121 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      181 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      241 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      301 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      361 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacact agtcgcaaaa
      421 aaccccgccc ctgacagggc ggggtttttt cgcggtctcc ccggggaggc gtcttcggat
      481 ccctcgagga agacgccgct aattggagac caccggtgcc gctacagggc gcgtcaggtg
      541 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      601 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      661 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      721 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      781 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      841 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      901 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      961 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1021 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1081 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1141 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1201 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1261 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1321 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1381 gctctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1441 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1501 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1561 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1621 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt agatctcatg
     1681 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1741 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1801 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1861 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1921 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1981 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2041 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2101 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2161 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacag
//
