LOCUS       sequence-177328-        6050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Flag-MCPIP1(150-599).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M
  TITLE     MCP-induced protein 1 deubiquitinates TRAF proteins and negatively 
            regulates JNK and NF-kappaB signaling.
  JOURNAL   J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. 
            Epub 2010 Nov 29.
  PUBMED    21115689
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20092641"; journalName: "J Exp Med"; date: "2010-12-20-
            20"; volume: "207"; issue: "13"; pages: "2959-73"
COMMENT     Sequence Label: Flag-MCPIP1(150-599)
FEATURES             Location/Qualifiers
     source          1..6050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             645..668
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2074..2696
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2273..2292)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2720..2740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2725..3054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2905..3040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3092..3111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3093..3111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3125..3141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3125..3141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3138..3160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3149..3165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3173..3179,3180..3197,3198..3203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3218..3239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3356..3373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3527..4115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3607..3626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4286..5077,5078..5146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4909..4928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5147..5251)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5278..5733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5365..5384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5575..5596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5859..5881
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5873..5890
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5874..5890
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattgatc taccatggcc cacaaccacc gccacaagca cgccgactac aaagacgatg
      661 acgacaagct tgcggccgcg aattcaatga acaaggaggt cttctcctgc cggggcatcc
      721 tgctggcagt gaactggttt ctggagcggg gccacacaga catcacagtg tttgtgccat
      781 cctggaggaa ggagcagcct cggcccgacg tgcccatcac agaccagcac atcctgcggg
      841 aactggagaa gaagaagatc ctggtgttca caccatcacg acgcgtgggt ggcaagcggg
      901 tggtgtgcta tgacgacaga ttcattgtga agctggccta cgagtctgac gggatcgtgg
      961 tttccaacga cacataccgt gacctccaag gcgagcggca ggagtggaag cgcttcatcg
     1021 aggagcggct gctcatgtac tccttcgtca atgacaagtt tatgccccct gatgacccac
     1081 tgggccggca cgggcccagc ctggacaact tcctgcgtaa gaagccactc actttggagc
     1141 acaggaagca gccgtgtccc tatggaagga aatgcaccta tgggatcaag tgccgattct
     1201 tccacccaga gcggccaagc tgcccccagc gctctgtggc agatgagctc cgtgccaatg
     1261 ctctcctctc accccccaga gccccaagca aggacaaaaa tggccggcgg ccttcacctt
     1321 catcccagtc cagctctctg ctaacagaga gtgagcagtg cagcctggat gggaagaagc
     1381 tgggggccca ggcatcccca gggtcccgcc aagagggtct aacacagacc tatgccccat
     1441 caggcaggag cctcgcacct agcgggggca gtggcagcag ctttgggccc acagactggc
     1501 tcccacagac gctggactca ctcccgtacg tctcccagga ttgcctggac tcgggcattg
     1561 gctccctgga gagccagatg tcggaacttt ggggggttcg aggaggaggc cctggtgagc
     1621 cgggcccacc ccgagcccct tacacgggct acagtcccta tggatctgag ctcccagcca
     1681 ccgcagcctt ctctgccttt ggccgggcca tgggtgctgg ccacttcagt gtccctgccg
     1741 actacccacc cgcgccccct gcctttccac ctcgagagta ctggtctgaa ccatacccac
     1801 tgcccccacc cacatcagtc cttcaggagc ccccagtgca gagcccaggg gctggcagga
     1861 gcccgtgggg cagggcaggc agcctggcca aggagcaggc cagcgtgtat actaagctgt
     1921 gtggtgtgtt tcccccgcac ctggtggagg ctgtgatggg gcgcttccca cagctcctgg
     1981 acccccagca gctggctgcc gagatcctct cctacaagtc ccagcacccc agtgagtaat
     2041 ctagatatcg gtaccggatc cagatctgat cccgggtggc atccctgtga cccctcccca
     2101 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     2161 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     2221 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     2281 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     2341 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     2401 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     2461 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     2521 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     2581 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     2641 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     2701 acgcggccag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2881 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2941 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3001 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     3061 ctcgaggaac tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa
     3121 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5281 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5341 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5401 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5461 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5521 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5581 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5641 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5701 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
     5761 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     5821 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     5881 gacggccagt gccaagctga tctatacatt gaatcaatat tggcaattag ccatattagt
     5941 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
     6001 tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
//
