LOCUS       sequence-177341-        3328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISDHFR
FEATURES             Location/Qualifiers
     source          1..3328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(760..778)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      774..821
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        886..977
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1842..1881
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1912..2500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2401..2420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2654..2671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2686..2825
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2751..2770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2911..2933
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2927..3118)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgggcag cagccatcat catcatcatc acagcagcgg
      181 atccatcagt ctgattgcgg cgctagcggt agatcgcgtt atcggcatgg aaaacgccat
      241 gccatggaac ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa ataaacccgt
      301 gattatgggg cgccatacct gggaatcaat cggtaggcct ttgcccggcc gcaaaaatat
      361 tatcctcagc agtcaacccg ggaccgatga tcgggttacc tgggttaaat cggtcgacga
      421 agccatcgcg gcgtgtggtg acgtaccaga aatcatggtg attggcggcg gacgcgttta
      481 tgaacagttc ttgccaaaag cgcaaaaact gtatctgacg catatcgatg cagaagtgga
      541 aggcgacacc cattttccgg attacgagcc ggatgactgg gaatcggtat tcagcgaatt
      601 ccacgatgct gatgcgcaga actcgcatag ctattgtttc gaaatcctcg agcgtcgtgc
      661 cggctaatgt acaagatcca gatctaagct tggtaccacg cgtgcgcgct gatccggctg
      721 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      781 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      841 cgggattagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct
      901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      961 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1021 cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag
     1081 aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc
     1141 ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat
     1201 gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact
     1261 attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa
     1321 tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact
     1381 tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag
     1441 accatgtaac tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg
     1501 agcgagacac cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg
     1561 aactacttac tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg
     1621 caggaccact actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg
     1681 caggagagcg tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc
     1741 gtatcgtagt tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga
     1801 ttgctgaaat aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct
     1861 tttggagcct ttttttttgg acggaccggt agaaaagatc aaaggatctt cttgagatcc
     1921 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1981 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2041 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2101 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2161 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2221 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2281 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2341 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2401 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2461 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2521 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2581 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2641 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     2701 tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc
     2761 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     2821 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2881 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     2941 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     3001 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     3061 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     3121 atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga aacgagagag
     3181 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     3241 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     3301 gcgcttcgtt aatacagatg taggtgtt
//
