LOCUS       sequence-177345-        3334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HPCDHFR
FEATURES             Location/Qualifiers
     source          1..3334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..184
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     primer_bind     complement(766..784)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      780..827
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        892..983
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1848..1887
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1918..2506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2407..2426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2660..2677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2692..2831
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2757..2776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2917..2939
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2933..3124)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgtctga agatcaggta gatccacgtt taatcgacgg
      181 taaaggatcc atcagtctga ttgcggcgct agcggtagat cgcgttatcg gcatggaaaa
      241 cgccatgcca tggaacctgc ctgccgatct cgcctggttt aaacgcaaca ccttaaataa
      301 acccgtgatt atggggcgcc atacctggga atcaatcggt aggcctttgc ccggccgcaa
      361 aaatattatc ctcagcagtc aacccgggac cgatgatcgg gttacctggg ttaaatcggt
      421 cgacgaagcc atcgcggcgt gtggtgacgt accagaaatc atggtgattg gcggcggacg
      481 cgtttatgaa cagttcttgc caaaagcgca aaaactgtat ctgacgcata tcgatgcaga
      541 agtggaaggc gacacccatt ttccggatta cgagccggat gactgggaat cggtattcag
      601 cgaattccac gatgctgatg cgcagaactc gcatagctat tgtttcgaaa tcctcgagcg
      661 tcgtgccggc taatgtacaa gatccagatc taagcttggt accacgcgtg cgcgctgatc
      721 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      781 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      841 ctatatcggg attagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
      901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctcgtg aaagtaaaag
     1081 acgcagagga ccaattgggg gcacgagtgg gatacataga actggacttg aatagcggta
     1141 aaatccttga gagttttcgc cctgaagagc gttttccaat gatgagcact ttcaaagttc
     1201 tgctatgtgg agcagtatta tcccgtgtag atgcggggca agagcaactc ggacgacgaa
     1261 tacactattc gcagaatgac ttggttgaat actccccagt gacagaaaag caccttacgg
     1321 acggaatgac ggtaagagaa ttatgtagtg ccgccataac gatgagtgat aacactgcgg
     1381 cgaacttact tctgacaacc atcggtggac cgaaggaatt aaccgctttt ttgcacaata
     1441 tgggagacca tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa gccataccaa
     1501 acgacgagcg agacaccaca atgcctgcgg caatggcaac aacattacgc aaactattaa
     1561 ctggcgaact acttactctg gcttcacggc aacaattaat agactggctt gaagcggata
     1621 aagttgcagg accactactg cgttcggcac ttcctgctgg ctggtttatt gctgataaat
     1681 ctggggcagg agagcgtggt tcacggggta tcattgccgc acttggacca gatggtaagc
     1741 cttcccgtat cgtagttatc tacacgacgg gtagtcaggc aactatggac gaacgaaata
     1801 gacagattgc tgaaataggg gcttcactga ttaagcattg gtaaaccgat acaattaaag
     1861 gctccttttg gagccttttt ttttggacgg accggtagaa aagatcaaag gatcttcttg
     1921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2041 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2701 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     2761 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2821 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2941 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     3001 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     3061 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     3121 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg gtaatgatac cgatgaaacg
     3181 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     3241 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
     3301 atgccagcgc ttcgttaata cagatgtagg tgtt
//
