LOCUS       sequence-177346-        3367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..3367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(799..817)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      813..860
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        925..1016
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1881..1920
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1951..2539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2440..2459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2693..2710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2725..2864
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2790..2809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2950..2972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2966..3157)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatgggcag cagccatcat catcatcatc acagcagcgg
      181 atctggtggt ggtggtggtg aaaacctgta cttccaggga tccatcagtc tgattgcggc
      241 gctagcggta gatcgcgtta tcggcatgga aaacgccatg ccatggaacc tgcctgccga
      301 tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatggggc gccatacctg
      361 ggaatcaatc ggtaggcctt tgcccggccg caaaaatatt atcctcagca gtcaacccgg
      421 gaccgatgat cgggttacct gggttaaatc ggtcgacgaa gccatcgcgg cgtgtggtga
      481 cgtaccagaa atcatggtga ttggcggcgg acgcgtttat gaacagttct tgccaaaagc
      541 gcaaaaactg tatctgacgc atatcgatgc agaagtggaa ggcgacaccc attttccgga
      601 ttacgagccg gatgactggg aatcggtatt cagcgaattc cacgatgctg atgcgcagaa
      661 ctcgcatagc tattgtttcg aaatcctcga gcgtcgtgcc ggctaatgta caagatccag
      721 atctaagctt ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag
      781 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      841 gggtcttgag gggttttttg ctgaaaggag gaactatatc gggattagcc cgcctaatga
      901 gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc
      961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1081 ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag
     1141 tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag
     1201 agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg
     1261 tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg
     1321 aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta
     1381 gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg
     1441 gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc
     1501 gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg
     1561 cggcaatggc aacaacatta cgcaaactat taactggcga actacttact ctggcttcac
     1621 ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg
     1681 cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg
     1741 gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga
     1801 cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac
     1861 tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     1921 cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2701 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2761 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2821 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     2881 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2941 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3001 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     3061 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     3121 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     3181 ttctgttcat ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     3241 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     3301 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     3361 aggtgtt
//
