LOCUS       sequence-177376-        3286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..3286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(718..736)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      732..779
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        844..935
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1800..1839
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1870..2458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2359..2378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2612..2629
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2644..2783
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2709..2728)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2869..2891
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2885..3076)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcaataat tttgtttaac tttaagaagg
      121 agatatacat atgggatcca tcagtctgat tgcggcgcta gcggtagatc gcgttatcgg
      181 catggaaaac gccatgccat ggaacctgcc tgccgatctc gcctggttta aacgcaacac
      241 cttaaataaa cccgtgatta tggggcgcca tacctgggaa tcaatcggta ggcctttgcc
      301 cggccgcaaa aatattatcc tcagcagtca acccgggacc gatgatcggg ttacctgggt
      361 taaatcggtc gacgaagcca tcgcggcgtg tggtgacgta ccagaaatca tggtgattgg
      421 cggcggacgc gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat
      481 cgatgcagaa gtggaaggcg acacccattt tccggattac gagccggatg actgggaatc
      541 ggtattcagc gaattccacg atgctgatgc gcagaactcg catagctatt gtttcgaaat
      601 cctcgagcgt cgtgccggca ccgaattttg cgcttaatgt acaaagcttg gtaccacgcg
      661 tgcgcgctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      721 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      781 tgaaaggagg aactatatcg ggattagccc gcctaatgag cgggcttttt tttaattccc
      841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      901 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      961 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctcg
     1021 tgaaagtaaa agacgcagag gaccaattgg gggcacgagt gggatacata gaactggact
     1081 tgaatagcgg taaaatcctt gagagttttc gccctgaaga gcgttttcca atgatgagca
     1141 ctttcaaagt tctgctatgt ggagcagtat tatcccgtgt agatgcgggg caagagcaac
     1201 tcggacgacg aatacactat tcgcagaatg acttggttga atactcccca gtgacagaaa
     1261 agcaccttac ggacggaatg acggtaagag aattatgtag tgccgccata acgatgagtg
     1321 ataacactgc ggcgaactta cttctgacaa ccatcggtgg accgaaggaa ttaaccgctt
     1381 ttttgcacaa tatgggagac catgtaactc gccttgaccg ttgggaacca gaactgaatg
     1441 aagccatacc aaacgacgag cgagacacca caatgcctgc ggcaatggca acaacattac
     1501 gcaaactatt aactggcgaa ctacttactc tggcttcacg gcaacaatta atagactggc
     1561 ttgaagcgga taaagttgca ggaccactac tgcgttcggc acttcctgct ggctggttta
     1621 ttgctgataa atctggggca ggagagcgtg gttcacgggg tatcattgcc gcacttggac
     1681 cagatggtaa gccttcccgt atcgtagtta tctacacgac gggtagtcag gcaactatgg
     1741 acgaacgaaa tagacagatt gctgaaatag gggcttcact gattaagcat tggtaaaccg
     1801 atacaattaa aggctccttt tggagccttt ttttttggac ggaccggtag aaaagatcaa
     1861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1981 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2461 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2521 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2641 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
     2701 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     2761 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     2821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     2941 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     3001 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     3061 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg gggtaatgat
     3121 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3181 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3241 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgtt
//
