LOCUS       sequence-17738-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Storz P, Doppler H, Toker A
  TITLE     Protein kinase Cdelta selectively regulates protein kinase 
            D-dependent activation of NF-kappaB in oxidative stress signaling.
  JOURNAL   Mol Cell Biol. 2004 Apr . 24(7):2614-26.
  PUBMED    15024053
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Apr . 24(7):2614-26."
COMMENT     Sequence Label: pSUPER.PKCdelta.RNAi
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..231
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     152..171
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(304..320)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(305..321)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(349..367)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(388..404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(388..404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(401..423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(436..442,443..460,461..466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    481..502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgggctgcag gaattcgaac gctgacgtca tcaacccgct ccaaggaatc gcgggcccag
       61 tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc aggaagatgg ctgtgtggga
      121 caggggtgtg gcgccctgca atatttgcat gtcgctatgt gttctgggaa atcaccataa
      181 acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca cagatccccg
      241 acaagatcat cggcagattt caagagaatc tgccgatgat cttgtctttt tggaaaagct
      301 tatcgatacc gtcgacctcg agggggggcc cggtaccagc ttttgttccc tttagtgagg
      361 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      601 tgggcgctct tccgcttcct cgctcactga tcgctgcgct cggtcttcgc tgcggcga
//
