LOCUS       sequence-177381-        3292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-HPCDHFR
FEATURES             Location/Qualifiers
     source          1..3292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             89..111
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             125..160
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     primer_bind     complement(724..742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      738..785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        850..941
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1806..1845
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1876..2464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2365..2384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2618..2635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2650..2789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2715..2734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2875..2897
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2891..3082)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tcaataattt tgtttaactt taagaaggag atatacatat
      121 gtctgaagat caggtagatc cacgtttaat cgacggtaaa ggatccatca gtctgattgc
      181 ggcgctagcg gtagatcgcg ttatcggcat ggaaaacgcc atgccatgga acctgcctgc
      241 cgatctcgcc tggtttaaac gcaacacctt aaataaaccc gtgattatgg ggcgccatac
      301 ctgggaatca atcggtaggc ctttgcccgg ccgcaaaaat attatcctca gcagtcaacc
      361 cgggaccgat gatcgggtta cctgggttaa atcggtcgac gaagccatcg cggcgtgtgg
      421 tgacgtacca gaaatcatgg tgattggcgg cggacgcgtt tatgaacagt tcttgccaaa
      481 agcgcaaaaa ctgtatctga cgcatatcga tgcagaagtg gaaggcgaca cccattttcc
      541 ggattacgag ccggatgact gggaatcggt attcagcgaa ttccacgatg ctgatgcgca
      601 gaactcgcat agctattgtt tcgaaatcct cgagcgtcgt gccggctaat gtacaggtac
      661 cacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      721 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      781 ttttgctgaa aggaggaact atatcgggat tagcccgcct aatgagcggg ctttttttta
      841 attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1021 cgctcgtgaa agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac
     1081 tggacttgaa tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga
     1141 tgagcacttt caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag
     1201 agcaactcgg acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga
     1261 cagaaaagca ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga
     1321 tgagtgataa cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa
     1381 ccgctttttt gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac
     1441 tgaatgaagc cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa
     1501 cattacgcaa actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag
     1561 actggcttga agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct
     1621 ggtttattgc tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac
     1681 ttggaccaga tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa
     1741 ctatggacga acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt
     1801 aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa
     1861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1981 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2641 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     2701 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     2761 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     2821 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2881 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     2941 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     3001 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     3061 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggt
     3121 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     3181 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     3241 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg tt
//
