LOCUS       sequence-177395-        3274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-HISDHFR
FEATURES             Location/Qualifiers
     source          1..3274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             83..105
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             125..142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(706..724)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      720..767
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        832..923
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1788..1827
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1858..2446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2347..2366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2600..2617
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2632..2771
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2697..2716)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2857..2879
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2873..3064)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggaaata attttgttta actttaagaa ggagatatac atatgggcag
      121 cagccatcat catcatcatc acagcagcgg atccatcagt ctgattgcgg cgctagcggt
      181 agatcgcgtt atcggcatgg aaaacgccat gccatggaac ctgcctgccg atctcgcctg
      241 gtttaaacgc aacaccttaa ataaacccgt gattatgggg cgccatacct gggaatcaat
      301 cggtaggcct ttgcccggcc gcaaaaatat tatcctcagc agtcaacccg ggaccgatga
      361 tcgggttacc tgggttaaat cggtcgacga agccatcgcg gcgtgtggtg acgtaccaga
      421 aatcatggtg attggcggcg gacgcgttta tgaacagttc ttgccaaaag cgcaaaaact
      481 gtatctgacg catatcgatg cagaagtgga aggcgacacc cattttccgg attacgagcc
      541 ggatgactgg gaatcggtat tcagcgaatt ccacgatgct gatgcgcaga actcgcatag
      601 ctattgtttc gaaatcctcg agcgtcgtgc cggctaatgt acaacgcgtg cgcgctgatc
      661 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      721 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      781 ctatatcggg attagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
      841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctcgtg aaagtaaaag
     1021 acgcagagga ccaattgggg gcacgagtgg gatacataga actggacttg aatagcggta
     1081 aaatccttga gagttttcgc cctgaagagc gttttccaat gatgagcact ttcaaagttc
     1141 tgctatgtgg agcagtatta tcccgtgtag atgcggggca agagcaactc ggacgacgaa
     1201 tacactattc gcagaatgac ttggttgaat actccccagt gacagaaaag caccttacgg
     1261 acggaatgac ggtaagagaa ttatgtagtg ccgccataac gatgagtgat aacactgcgg
     1321 cgaacttact tctgacaacc atcggtggac cgaaggaatt aaccgctttt ttgcacaata
     1381 tgggagacca tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa gccataccaa
     1441 acgacgagcg agacaccaca atgcctgcgg caatggcaac aacattacgc aaactattaa
     1501 ctggcgaact acttactctg gcttcacggc aacaattaat agactggctt gaagcggata
     1561 aagttgcagg accactactg cgttcggcac ttcctgctgg ctggtttatt gctgataaat
     1621 ctggggcagg agagcgtggt tcacggggta tcattgccgc acttggacca gatggtaagc
     1681 cttcccgtat cgtagttatc tacacgacgg gtagtcaggc aactatggac gaacgaaata
     1741 gacagattgc tgaaataggg gcttcactga ttaagcattg gtaaaccgat acaattaaag
     1801 gctccttttg gagccttttt ttttggacgg accggtagaa aagatcaaag gatcttcttg
     1861 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1921 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1981 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2041 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2101 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2221 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2401 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2461 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2521 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2581 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2641 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     2701 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2761 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2821 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2881 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     2941 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     3001 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     3061 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg gtaatgatac cgatgaaacg
     3121 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     3181 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
     3241 atgccagcgc ttcgttaata cagatgtagg tgtt
//
