LOCUS       sequence-177411-        3256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..3256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             83..105
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(688..706)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      702..749
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        814..905
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1770..1809
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1840..2428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2329..2348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2582..2599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2614..2753
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2679..2698)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2839..2861
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2855..3046)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggaaata attttgttta actttaagaa ggagatatac atatgggatc
      121 catcagtctg attgcggcgc tagcggtaga tcgcgttatc ggcatggaaa acgccatgcc
      181 atggaacctg cctgccgatc tcgcctggtt taaacgcaac accttaaata aacccgtgat
      241 tatggggcgc catacctggg aatcaatcgg taggcctttg cccggccgca aaaatattat
      301 cctcagcagt caacccggga ccgatgatcg ggttacctgg gttaaatcgg tcgacgaagc
      361 catcgcggcg tgtggtgacg taccagaaat catggtgatt ggcggcggac gcgtttatga
      421 acagttcttg ccaaaagcgc aaaaactgta tctgacgcat atcgatgcag aagtggaagg
      481 cgacacccat tttccggatt acgagccgga tgactgggaa tcggtattca gcgaattcca
      541 cgatgctgat gcgcagaact cgcatagcta ttgtttcgaa atcctcgagc gtcgtgccgg
      601 caccgaattt tgcgcttaat gtacaacgcg tgcgcgctga tccggctgct aacaaagccc
      661 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      721 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcg ggattagccc
      781 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg
     1021 gggcacgagt gggatacata gaactggact tgaatagcgg taaaatcctt gagagttttc
     1081 gccctgaaga gcgttttcca atgatgagca ctttcaaagt tctgctatgt ggagcagtat
     1141 tatcccgtgt agatgcgggg caagagcaac tcggacgacg aatacactat tcgcagaatg
     1201 acttggttga atactcccca gtgacagaaa agcaccttac ggacggaatg acggtaagag
     1261 aattatgtag tgccgccata acgatgagtg ataacactgc ggcgaactta cttctgacaa
     1321 ccatcggtgg accgaaggaa ttaaccgctt ttttgcacaa tatgggagac catgtaactc
     1381 gccttgaccg ttgggaacca gaactgaatg aagccatacc aaacgacgag cgagacacca
     1441 caatgcctgc ggcaatggca acaacattac gcaaactatt aactggcgaa ctacttactc
     1501 tggcttcacg gcaacaatta atagactggc ttgaagcgga taaagttgca ggaccactac
     1561 tgcgttcggc acttcctgct ggctggttta ttgctgataa atctggggca ggagagcgtg
     1621 gttcacgggg tatcattgcc gcacttggac cagatggtaa gccttcccgt atcgtagtta
     1681 tctacacgac gggtagtcag gcaactatgg acgaacgaaa tagacagatt gctgaaatag
     1741 gggcttcact gattaagcat tggtaaaccg atacaattaa aggctccttt tggagccttt
     1801 ttttttggac ggaccggtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1981 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2521 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2581 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2641 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2701 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2761 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2821 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2881 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2941 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     3001 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     3061 aagggggatt tctgttcatg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     3121 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     3181 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     3241 tacagatgta ggtgtt
//
