LOCUS       sequence-177415-        2839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  original polycistronic cloning vector for plasmid suite (generation 
            I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST39
FEATURES             Location/Qualifiers
     source          1..2839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     102..119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     103..119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(269..287)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      283..330
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        397..488
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1353..1392
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1423..2011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1912..1931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2165..2182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2197..2336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2262..2281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2422..2444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2438..2629)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctctaga aggcctcgga tccgggccct gtacaagatc ctgtaaaacg acggccagtg
      121 aattccccgg gaagcttcgc cagggttttc ccagtcgagc tcgatatcgg taccagcgga
      181 taacaatttc acatccggat tcgcgaacgc gtctcgagag atccggctgc taacaaagcc
      241 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      301 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cgactagtag
      361 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
      421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      541 gccttcctgt ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca gaggaccaat
      601 tgggggcacg agtgggatac atagaactgg acttgaatag cggtaaaatc cttgagagtt
      661 ttcgccctga agagcgtttt ccaatgatga gcactttcaa agttctgcta tgtggagcag
      721 tattatcccg tgtagatgcg gggcaagagc aactcggacg acgaatacac tattcgcaga
      781 atgacttggt tgaatactcc ccagtgacag aaaagcacct tacggacgga atgacggtaa
      841 gagaattatg tagtgccgcc ataacgatga gtgataacac tgcggcgaac ttacttctga
      901 caaccatcgg tggaccgaag gaattaaccg cttttttgca caatatggga gaccatgtaa
      961 ctcgccttga ccgttgggaa ccagaactga atgaagccat accaaacgac gagcgagaca
     1021 ccacaatgcc tgcggcaatg gcaacaacat tacgcaaact attaactggc gaactactta
     1081 ctctggcttc acggcaacaa ttaatagact ggcttgaagc ggataaagtt gcaggaccac
     1141 tactgcgttc ggcacttcct gctggctggt ttattgctga taaatctggg gcaggagagc
     1201 gtggttcacg gggtatcatt gccgcacttg gaccagatgg taagccttcc cgtatcgtag
     1261 ttatctacac gacgggtagt caggcaacta tggacgaacg aaatagacag attgctgaaa
     1321 taggggcttc actgattaag cattggtaaa ccgatacaat taaaggctcc ttttggagcc
     1381 tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1561 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2101 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2161 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     2221 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     2281 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2341 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2461 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2521 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2581 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2641 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgagaga ggatgctcac
     2701 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     2761 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     2821 taatacagat gtaggtgtt
//
