LOCUS       sequence-177416-        5805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of mouse CSPG5 long isoform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5805)
  TITLE     Rathjen lab plasmids
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSG5-mCALEBlong (aa 1-566)
FEATURES             Location/Qualifiers
     source          1..5805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..35
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..101)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        189..399
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        211..407
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      258..393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          438..1010
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1018..1037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1065..1084
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1065..1083
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2855..2976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2892..2911)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2946..2965
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3010..3027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3181..3769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3261..3280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3940..4731,4732..4800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4563..4582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4801..4905)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4973..4991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5029..5051)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5151..5170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5237..5692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5374..5395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5586..5605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg tcgacttctg
       61 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaagga aagtccccag
      181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      361 tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag ctccggatcg
      421 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
      481 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
      541 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
      601 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
      661 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
      721 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
      781 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
      841 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
      901 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
      961 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1021 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     1081 agggcgaatt cgcgcaccgg caacagcggc aggggctccg cgcaatgggc cgagctggag
     1141 gcgggggccc ggactggggg ccgccgccag tgctgctgct tctgggggtc acgctggtgc
     1201 tcaccgctgg ggccgtaccg gcacgggaaa caggcagtgc gatcgaggct gaagagctgg
     1261 tgaggagcag cctggcatgg gagtcacgtg ccaatgacac gcgggaggaa gccggcctgc
     1321 cagcagctgg ggaagatgag acctcgtgga cagagcgggg cagtgagatg gctgcggtgg
     1381 gccctggggt cgggccagag gaggcactag aggcatcggc tgcagtgact ggcactgcct
     1441 ggctagaggc agatggccca ggcctgggtg gagtgactgc agaggctggc agtggcgacg
     1501 cccagaccct tccagctacg ctccaggctc ctgatgaggc ccttgggtca tctacaatgc
     1561 cccctgccat ccctgaggct actgaaacca gtggacctcc ctcccctgct gtccatgata
     1621 agcctagtgt aggccctgaa ctccctaaag agatcccctt ggaggttcgg ctgaacctgg
     1681 gaggcagcac accagagccc acttttcccc ttcagggcac tctcgagacc caaccagcct
     1741 cagatataat tgacattgat tactttgaag gattggatag tgagggtcgt ggtgcagaca
     1801 tgggcagctt cccggggtca ccaggaacct cagaaaatca ccctgatacc gaaggagaga
     1861 ccccttcctg gagcctgctt gatttgtatg atgacttcac cccttttgat gagtctgatt
     1921 tctaccccac cacatccttc tatgatgatt tggaagagga ggaagaagag gaggaggata
     1981 aggatacagt aggaggtgga gacctggaag atgaaaacga ccttctcctg ccctctcaaa
     2041 agcctggtgt ggggcctggg acaggacagc ccaacaaccg gtggcatgct gttcccccac
     2101 agcatactct ggggatggta cctggcagca gcatctctct taggccccgc cccggagatc
     2161 caggcaagga cctggcctca ggagaaaatg gcacagagtg ccgagttggc ttcgtcaggc
     2221 acaatggctc ctgccggtca gtctgtgacc tctttccgag ttactgtcac aacggcggcc
     2281 agtgctacct ggtggagaac ataggggctt tctgcaggtg taacacccag gactacatct
     2341 ggcacaaggg gatgcgctgt gagtccatca tcacggactt ccaggtgatg tgcgtggccg
     2401 ttggctcggc tgctctcgtg cttctcctcc tgttcatgat gactgtgttc tttgccaaga
     2461 agctctatct gctcaagact gagaatacca agctgcggag gaccaataaa ttccggaccc
     2521 catctgagct ccacaacgac aacttctccc tctccaccat tgccgagggc tctcatccaa
     2581 atgtaaggaa attttgcgac actccccgtg tctcctcccc ccatgcccgt gccttggctc
     2641 actatgataa cattgtctgt caggacgacc ccagcgctcc ccacaaaatc caggaccctc
     2701 tcaagtcccg cctgaaggag gaagagtcct ttaacatcca gaactccatg tcacccaaac
     2761 ttgagggtgg caaaggtgac caggatgact tgggggtgaa ctgtctgcag aataacctaa
     2821 cctgagactg aggatccaga tcttattaaa gcagaacttg tttattgcag cttataatgg
     2881 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2941 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga ctctagactc
     3001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4921 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4981 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5041 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5161 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5221 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5281 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5341 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5401 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5461 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5521 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5581 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5641 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
     5701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5761 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaa
//
