LOCUS       sequence-177427-         441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISDHFR
FEATURES             Location/Qualifiers
     source          1..441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tagctatgcg agttctgcgc atcagcatcg tggaattcgc tgaataccga ttcccagtca
       61 tccggctcgt aatccggaaa atgggtgtcg ccttccactt ctgcatcgat atgcgtcaga
      121 tacagttttt gcgcttttgg caagaactgt tcataaacgc gtccgccgcc aatcaccatg
      181 atttctggta cgtcaccaca cgccgcgatg gcttcgtcga ccgatttaac ccaggtaacc
      241 cgatcatcgg tcccgggttg actgctgagg ataatatttt tgcggccggg caaaggccta
      301 ccgattgatt cccaggtatg gcgccccata atcacgggtt tatttaaggt gttgcgttta
      361 aaccaggcga gatcggcagg caggttccat ggcatggcgt tttccatgcc gataacgcga
      421 tctaccgcta gcgccgcaat c
//
