LOCUS       sequence-177428-         391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISDHFR
FEATURES             Location/Qualifiers
     source          1..391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..31
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatgggcagc agccatcatc atcatcatca cagcagcgga tccatcagtc tgattgcggc
       61 gctagcggta gatcgcgtta tcggcatgga aaacgccatg ccatggaacc tgcctgccga
      121 tctcgcctgg tttaaacgca acaccttaaa taaacccgtg attatggggc gccatacctg
      181 ggaatcaatc ggtaggcctt tgcccggccg caaaaatatt atcctcagca gtcaacccgg
      241 gaccgatgat cgggttacct gggttaaatc ggtcgacgaa gccatcgcgg cgtgtggtga
      301 cgtaccagaa atcatggtga ttggcggcgg acgcgtttat gaacagttct tgccaaaagc
      361 gcaaaaactg tatctgacgc atatcgatgc a
//
