LOCUS       sequence-177439-         482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HPCDHFR
FEATURES             Location/Qualifiers
     source          1..482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..43
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 tatgtctgaa gatcaggtag atccacgttt aatcgacggt aaaggatcca tcagtctgat
       61 tgcggcgcta gcggtagatc gcgttatcgg catggaaaac gccatgccat ggaacctgcc
      121 tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta tggggcgcca
      181 tacctgggaa tcaatcggta ggcctttgcc cggccgcaaa aatattatcc tcagcagtca
      241 acccgggacc gatgatcggg ttacctgggt taaatcggtc gacgaagcca tcgcggcgtg
      301 tggtgacgta ccagaaatca tggtgattgg cggcggacgc gtttatgaac agttcttgcc
      361 aaaagcgcaa aaactgtatc tgacgcatat cgatgcagaa gtggaaggcg acacccattt
      421 tccggattac gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc
      481 gc
//
