LOCUS       sequence-177446-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(536..556)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(584..601)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             621..643
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggatcagcg cgcacgcgtg gtaccaagct tagatctgga tcttgtacat tagccggcac
       61 gacgctcgag gatttcgaaa caatagctat gcgagttctg cgcatcagca tcgtggaatt
      121 cgctgaatac cgattcccag tcatccggct cgtaatccgg aaaatgggtg tcgccttcca
      181 cttctgcatc gatatgcgtc agatacagtt tttgcgcttt tggcaagaac tgttcataaa
      241 cgcgtccgcc gccaatcacc atgatttctg gtacgtcacc acacgccgcg atggcttcgt
      301 cgaccgattt aacccaggta acccgatcat cggtcccggg ttgactgctg aggataatat
      361 ttttgcggcc gggcaaaggc ctaccgattg attcccaggt atggcgcccc ataatcacgg
      421 gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc catggcatgg
      481 cgttttccat gccgataacg cgatctaccg ctagcgccgc aatcagactg atggatccct
      541 ggaagtacag gttttcacca ccaccaccac cagatccgct gctgtgatga tgatgatgat
      601 ggctgctgcc catatgtata tctccttctt aaagttaaac aaaattattt ctagagctag
      661 cgaattccaa ttggagctct ccggagccgg agggaaaccg ttgtggtctc cctatagtga
      721 gtcg
//
