LOCUS       sequence-177447-         523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HISNDHFR
FEATURES             Location/Qualifiers
     source          1..523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atatacatat gggcagcagc catcatcatc atcatcacag cagcggatct ggtggtggtg
       61 gtggtgaaaa cctgtacttc cagggatcca tcagtctgat tgcggcgcta gcggtagatc
      121 gcgttatcgg catggaaaac gccatgccat ggaacctgcc tgccgatctc gcctggttta
      181 aacgcaacac cttaaataaa cccgtgatta tggggcgcca tacctgggaa tcaatcggta
      241 ggcctttgcc cggccgcaaa aatattatcc tcagcagtca acccgggacc gatgatcggg
      301 ttacctgggt taaatcggtc gacgaagcca tcgcggcgtg tggtgacgta ccagaaatca
      361 tggtgattgg cggcggacgc gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc
      421 tgacgcatat cgatgcagaa gtggaaggcg acacccattt tccggattac gagccggatg
      481 actgggaatc ggtattcagc gaattccacg atgctgatgc gca
//
