LOCUS       sequence-177449-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-FLAGNDHFR
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(444..464)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(486..509)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             523..545
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gagttctgcg catcagcatc gtggaattcg ctgaataccg attcccagtc atccggctcg
       61 taatccggaa aatgggtgtc gccttccact tctgcatcga tatgcgtcag atacagtttt
      121 tgcgcttttg gcaagaactg ttcataaacg cgtccgccgc caatcaccat gatttctggt
      181 acgtcaccac acgccgcgat ggcttcgtcg accgatttaa cccaggtaac ccgatcatcg
      241 gtcccgggtt gactgctgag gataatattt ttgcggccgg gcaaaggcct accgattgat
      301 tcccaggtat ggcgccccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg
      361 agatcggcag gcaggttcca tggcatggcg ttttccatgc cgataacgcg atctaccgct
      421 agcgccgcaa tcagactgat ggatccctgg aagtacaggt tttcaccacc accaccacca
      481 gatcccttgt catcatcgtc cttgtagtct cccatatgta tatctccttc ttaaagttaa
      541 acaaaattat ttctagagct agcgaattcc aattggagct ctccggagcc ggagggaaa
//
