LOCUS       sequence-177450-         392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HPCNDHFR
FEATURES             Location/Qualifiers
     source          1..392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(336..356)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agatacagtt tttgcgcttt tggcaagaac tgttcataaa cgcgtccgcc gccaatcacc
       61 atgatttctg gtacgtcacc acacgccgcg atggcttcgt cgaccgattt aacccaggta
      121 acccgatcat cggtcccggg ttgactgctg aggataatat ttttgcggcc gggcaaaggc
      181 ctaccgattg attcccaggt atggcgcccc ataatcacgg gtttatttaa ggtgttgcgt
      241 ttaaaccagg cgagatcggc aggcaggttc catggcatgg cgttttccat gccgataacg
      301 cgatctaccg ctagcgccgc aatcagactg atggatccct ggaagtacag gttttcacca
      361 ccaccaccac cagatccttt accgtcgatt aa
//
