LOCUS       sequence-177454-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-CBPHISNDHFR
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(549..569)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(597..614)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(627..704)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             718..740
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(807..826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(808..826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttgttagc agccggatca gcgcgcacgc gtggtaccaa gcttagatct ggatcttgta
       61 cattagccgg cacgacgctc gaggatttcg aaacaatagc tatgcgagtt ctgcgcatca
      121 gcatcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc cggaaaatgg
      181 gtgtcgcctt ccacttctgc atcgatatgc gtcagataca gtttttgcgc ttttggcaag
      241 aactgttcat aaacgcgtcc gccgccaatc accatgattt ctggtacgtc accacacgcc
      301 gcgatggctt cgtcgaccga tttaacccag gtaacccgat catcggtccc gggttgactg
      361 ctgaggataa tatttttgcg gccgggcaaa ggcctaccga ttgattccca ggtatggcgc
      421 cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg
      481 ttccatggca tggcgttttc catgccgata acgcgatcta ccgctagcgc cgcaatcaga
      541 ctgatggatc cctggaagta caggttttca ccaccaccac caccagatcc gctgctgtga
      601 tgatgatgat gatggctaga tcccagaagt gccccggagg atgagatttt cttaaagcgg
      661 ttggctgctg agacggctat gaaattcttt ttccatcgtc gcttagacat atgtatatct
      721 ccttcttaaa gttaaacaaa attatttcta gagctagcga attccaattg gagctctccg
      781 gagccggagg gaaaccgttg tggtctccct atagtgagtc gtattaattt cgcgggatcg
      841 atgcatggtg ccaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
      901 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
      961 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     1021 cattacccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa
     1081 accgccctta acatggcccg ctttatcaga agccagacat taa
//
