LOCUS       sequence-177468-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             517..539
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(606..625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(607..625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cattaagcgc aaaattcggt gccggcacga cgctcgagga tttcgaaaca atagctatgc
       61 gagttctgcg catcagcatc gtggaattcg ctgaataccg attcccagtc atccggctcg
      121 taatccggaa aatgggtgtc gccttccact tctgcatcga tatgcgtcag atacagtttt
      181 tgcgcttttg gcaagaactg ttcataaacg cgtccgccgc caatcaccat gatttctggt
      241 acgtcaccac acgccgcgat ggcttcgtcg accgatttaa cccaggtaac ccgatcatcg
      301 gtcccgggtt gactgctgag gataatattt ttgcggccgg gcaaaggcct accgattgat
      361 tcccaggtat ggcgccccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg
      421 agatcggcag gcaggttcca tggcatggcg ttttccatgc cgataacgcg atctaccgct
      481 agcgccgcaa tcagactgat ggatcccata tgtatatctc cttcttaaag ttaaacaaaa
      541 ttatttctag agctagcgaa ttccaattgg agctctccgg agccggaggg aaaccgttgt
      601 ggtctcccta tagtgagtcg tattaatttc gcgggatcga tgcatggtgc caacacctac
      661 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
      721 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
      781 taac
//
