LOCUS       sequence-177473-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-CBPDHFR
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(493..570)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             584..606
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttagccggca cgacgctcga ggatttcgaa acaatagcta tgcgagttct gcgcatcagc
       61 atcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg gaaaatgggt
      121 gtcgccttcc acttctgcat cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa
      181 ctgttcataa acgcgtccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc
      241 gatggcttcg tcgaccgatt taacccaggt aacccgatca tcggtcccgg gttgactgct
      301 gaggataata tttttgcggc cgggcaaagg cctaccgatt gattcccagg tatggcgccc
      361 cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt
      421 ccatggcatg gcgttttcca tgccgataac gcgatctacc gctagcgccg caatcagact
      481 gatggatccc agaagtgccc cggaggatga gattttctta aagcggttgg ctgctgagac
      541 ggctatgaaa ttctttttcc atcgtcgctt agacatatgt atatctcctt cttaaagtta
      601 aacaaaatta ttgaattcca attggagctc tccggagccg gagggaaacc gttgtggtct
      661 ccctatagtg agtcgtatta atttcgcggg atcgatgcat ggtgccaaca cctacatctg
      721 tattaacgaa gcgctggcat tgaccctgag tgattttt
//
