LOCUS       sequence-177475-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-STRDHFR
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(478..501)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             518..540
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(595..614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(596..614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgctcgagg atttcgaaac aatagctatg cgagttctgc gcatcagcat cgtggaattc
       61 gctgaatacc gattcccagt catccggctc gtaatccgga aaatgggtgt cgccttccac
      121 ttctgcatcg atatgcgtca gatacagttt ttgcgctttt ggcaagaact gttcataaac
      181 gcgtccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcgtc
      241 gaccgattta acccaggtaa cccgatcatc ggtcccgggt tgactgctga ggataatatt
      301 tttgcggccg ggcaaaggcc taccgattga ttcccaggta tggcgcccca taatcacggg
      361 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc atggcatggc
      421 gttttccatg ccgataacgc gatctaccgc tagcgccgca atcagactga tggatccttt
      481 ctcgaactgc gggtggctcc agcttgccat atgtatatct ccttcttaaa gttaaacaaa
      541 attattgaat tccaattgga gctctccgga gccggaggga aaccgttgtg gtctccctat
      601 agtgagtcgt attaatttcg cgggatcgat gcatggtgcc aacacctaca tctgtattaa
      661 cgaagcgctg gcattgaccc tga
//
