LOCUS       sequence-177495-        7417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of chicken Contactin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7417)
  TITLE     Rathjen lab plasmids
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSG5-chContactin1
FEATURES             Location/Qualifiers
     source          1..7417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        152..362
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        174..370
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      221..356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          401..973
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     981..1000
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1028..1047
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1028..1046
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4430..4551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4467..4486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4521..4540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4585..4602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4756..5344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4836..4855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5515..6306,6307..6375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6138..6157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6376..6480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6548..6566
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6604..6626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6726..6745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6812..7267)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6949..6970)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7161..7180
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7393..7415
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7407..7417,1..7)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(7408..7417,1..7)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccagtgaa ttgtcgactt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      181 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      241 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      301 ggccgcctcg gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggcctagg
      361 cttttgcaaa aagctccgga tcgatcctga gaacttcagg gtgagtttgg ggacccttga
      421 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
      481 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
      541 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
      601 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
      661 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
      721 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
      781 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
      841 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
      901 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
      961 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     1021 agaattgtaa tacgactcac tatagggcga attcctttcg agataatctc tgaatagaag
     1081 ataaccatga ggttcttcat cagtcatctt gttacactct gtttcatctt ctgtgtggca
     1141 gactctaccc atttttcaga ggaaggaaac aaaggttatg gacccgtttt tgaggagcaa
     1201 cccattgata ctatctatcc agaagaatct tcagatggac aagtttcaat gaactgcagg
     1261 gctcgagcag ttccttttcc tacttacaag tggaaactca acaactggga tattgattta
     1321 acaaaggatc gctacagtat ggtaggaggc agacttgtta tcagtaatcc agagaaatca
     1381 agggatgctg gaaaatatgt ctgtgtagta tcgaatatct ttggaactgt cagaagcagt
     1441 gaagccactc tgagttttgg atatctggat ccttttccac cagaggaaca ctatgaggtt
     1501 aaagtacgag aaggtgttgg agcagtgctt ctttgtgagc ctccttacca ctacccagat
     1561 gatctcagtt atcgctggct tctaaatgag ttcccagtat tcattgcttt ggacagacga
     1621 cggtttgtgt ctcagacgaa tggcaatctc tacattgcaa atgtagaagc atcagataag
     1681 ggaaattatt catgttttgt gtccagccct tcgataacaa agagtgtatt cagtaaattt
     1741 attccactta ttccacaggc cgatcgtgcc aaagtgtatc ctgctgatat caaggtgaag
     1801 ttcaaggaca cgtatgctct cctggggcaa aatgtgacac tggagtgttt tgctcttggg
     1861 aatccggttc ctgaactcag atggagtaaa taccttgaac ctatgccagc cactgctgag
     1921 ataagcatgt ctggtgcagt tcttaagatc tttaatattc agtatgaaga tgaaggactt
     1981 tatgagtgtg aagctgaaaa ctataaagga aaagataaac atcaagcaag agtttatgtg
     2041 caagcctctc cagagtgggt agaacatatc aacgacacag agaaggatat aggcagcgat
     2101 ctctactggc cgtgtgtagc tacaggcaag cctattccaa caattaggtg gttgaagaac
     2161 ggtgtctcgt tccggaaagg tgaactacga attcaaggcc tgacctttga agatgctggc
     2221 atgtatcagt gtatagcaga aaacgcacat ggaattattt atgcaaatgc tgaactgaag
     2281 attgtggctt cacctcctac ttttgaactc aaccctatga agaagaaaat cctagcagct
     2341 aaaggggggc gtgtaatcat tgaatgcaaa cctaaagctg ctcctaagcc aaaattctcc
     2401 tggagcaaag gaacagaatt gcttgtaaat gggagccgca tacatatttg ggatgatggg
     2461 agcctggaaa ttatcaatgt cacaaagttg gatgaaggcc gctacacctg ttttgctgaa
     2521 aataaccgag gaaaagctaa tagcactggt gttctggaaa tgacagaagc tacaaggatt
     2581 actttagcac cactgaacgt tgatgtcact gttggagaga atgctactat gcaatgcatt
     2641 gcatcccatg atcccacatt agatctgaca tttatttggt ctctaaatgg ctttgtaata
     2701 gattttgaga aagagcacga acattatgaa aggaatgtca tgattaaatc caatggtgag
     2761 ctgctcatta aaaatgttca gctgcggcat gctggaagat acacatgtac tgcccagaca
     2821 attgttgaca attcttcagc ttcagctgac cttgttgtaa gaggtcctcc aggccctcca
     2881 ggaggtataa gaatagaaga aattagagac actgctgtag cacttacctg gagtcgtgga
     2941 acagacaatc atagccctat ttctaaatac actatccagt caaaaacttt cctatctgaa
     3001 gagtggaaag atgccaaaac agagccatca gatattgaag gaaacatgga atctgctaga
     3061 gtgattgatt taattccatg gatggaatat gagttccgga taatagcaac aaacactttg
     3121 ggaactgggg aaccgagtat gccatcacag agaatcagaa ctgaaggcgc tcctcctaat
     3181 gtggctcctt ctgatgttgg aggaggaggt ggaagcaatc gagagctgac gataacatgg
     3241 atgcctttgt caagagaata ccactatggc aataactttg gttacatagt ggctttcaag
     3301 ccgtttggtg agaaagaatg gagaagagtt acagttacta atcctgaaat tggccgttat
     3361 gtccacaagg atgaatcaat gcctccttcc acccagtacc aagtaaaagt gaaagccttt
     3421 aacagcaaag gggatggacc attcagtctc actgctgtca tttattcagc acaagatgct
     3481 ccaactgagg tacctacaga tgtgagcgtg aaagttttgt catcttctga aatttctgtt
     3541 tcttggcatc acgttactga gaaatctgtg gaaggatatc agattcgtta ctgggctgct
     3601 catgataaag aggcagctgc gcagagggtg caagtaagca atcaagagta ttcaaccaaa
     3661 ctggaaaact tgaagcctaa tacacgctac cacatagatg tttctgcctt caacagtgca
     3721 ggctatggac ctcccagtag aaccattgac attattacca ggaaagcacc tccaagccaa
     3781 cgtccaagaa tcatcagttc tgtaagatct ggttcaaggt acatcatcac ctgggatcat
     3841 gtgaaggcaa tgtcaaatga atccgcagtt gaaggataca aagtgctata tagaccagat
     3901 ggacaacatg aaggcaagtt gttttcaact ggaaaacaca caatagaggt cccagtcccc
     3961 agtgatggtg aatatgttgt tgaggtccga gcacacagtg aaggaggaga tggagaagta
     4021 gctcaaatta aaatttcagg tgccactgct ggagtaccga ctctgcttct gggcctggtg
     4081 ctgcctgccc tcggtgtcct tgcctactcg ggattctgaa tgtagccggg tttaccttgt
     4141 cctcacccga acacaaggga ctccctttga agaggaggac tgcaactctc gattcctgtg
     4201 gcaccatcct aagccaaata aattacactt taaaataagt taccctacca atttcctatg
     4261 gactttaaaa ggactgaggc atccaggaac tccttcatta agtaaatcaa cttttgaaag
     4321 gataagaaat atttctaact tgttccaata tgccttataa aaacacttat gctgatctgt
     4381 gacagttgca tgattcggcc gaattcggat ccagatctta ttaaagcaga acttgtttat
     4441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4561 gtcgactcta gactcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4621 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4681 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5161 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5821 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5881 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5941 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6001 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6061 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6121 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6181 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6241 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6301 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6361 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6421 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6481 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6541 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6601 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6661 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6721 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6781 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
     6841 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     6901 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     6961 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7021 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     7081 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     7141 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     7201 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     7261 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     7321 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     7381 cgccagggtt ttcccagtca cgacgttgta aaacgac
//
