LOCUS       sequence-177499-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-HPCHISNDHFR
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(539..559)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(587..604)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(614..649)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     RBS             663..685
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(740..759)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(741..759)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat cagcgcgcac gcgtggtacc aagctttgta cattagccgg
       61 cacgacgctc gaggatttcg aaacaatagc tatgcgagtt ctgcgcatca gcatcgtgga
      121 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cggaaaatgg gtgtcgcctt
      181 ccacttctgc atcgatatgc gtcagataca gtttttgcgc ttttggcaag aactgttcat
      241 aaacgcgtcc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
      301 cgtcgaccga tttaacccag gtaacccgat catcggtccc gggttgactg ctgaggataa
      361 tatttttgcg gccgggcaaa ggcctaccga ttgattccca ggtatggcgc cccataatca
      421 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccatggca
      481 tggcgttttc catgccgata acgcgatcta ccgctagcgc cgcaatcaga ctgatggatc
      541 cctggaagta caggttttca ccaccaccac caccagatcc gctgctgtga tgatgatgat
      601 gatggctaga tcctttaccg tcgattaaac gtggatctac ctgatcttca gacatatgta
      661 tatctccttc ttaaagttaa acaaaattat tgaattccaa ttggagctct ccggagccgg
      721 agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgatgcatg
      781 gtgccaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc
      841 tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca
      901 tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc
      961 ccatgaacag aaatccccct tacacgga
//
