LOCUS       sequence-177501-        8054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of chicken Contactin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8054)
  TITLE     Rathjen lab plasmids
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSG5-chContactin2
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..46
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     38..55
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     39..55
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(92..112)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        200..410
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        222..418
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      269..404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          449..1021
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1029..1048
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1076..1095
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1076..1094
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4319..4328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5115..5236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5152..5171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5206..5225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5270..5287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5441..6029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5521..5540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6200..6991,6992..7060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6823..6842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7061..7165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7233..7251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7289..7311)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7411..7430
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7497..7952)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7634..7655)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7846..7865
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
       61 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
      121 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg
      181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      361 ctctgagcta ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa
      421 gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
      481 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
      541 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
      601 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
      661 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
      721 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
      781 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
      841 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
      901 catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata
      961 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1021 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata
     1081 cgactcacta tagggcgaat tcggcacgag gccgggctca gcaccgcggc aggagctccg
     1141 cggccccgca ctcccccgcc tcatgctcag cccccgaccc tatcccaggg ctgctgtccc
     1201 cactcagcca tagagccacg gccataacat ctggagcaca ggatgggagg cactgctgca
     1261 ttcatctgca cctccctggc cgtcatcatc tgcgtggtct ggtgccaagc acagagtggc
     1321 atgaggagct acgggccggt gtttgaggag cagccagcac acaccctgtt ccctgaaggc
     1381 tcagcagaag agaaagtgac gctgacgtgt cgagccagag ccaacccccc tgcaacatac
     1441 agatggaaga tgaacggcac agagctgaag atggggccgg attcacggta caggttggtt
     1501 gctggggacc tggtgataag caacccagtg aaagcaaagg atgcaggctc ctaccagtgt
     1561 gtggcaacca acgcccgtgg cacagtggtc agcagggaag catccctccg ctttggcttt
     1621 ttgcaggaat tctctgcaga agagcgagac cccgtgaaga tcaccgaagg ttggggagtg
     1681 atgttcacct gcagcccccc tccccattac ccagccttgt cctaccggtg gcttctgaat
     1741 gagtttccca acttcatccc agccgacgga aggcgtttcg tctctcagac cacaggaaac
     1801 ctctacattg ccaagacaga ggcttccgac ctcggcaact actcgtgctt tgccaccagc
     1861 cacatcgact tcatcaccaa gagcgttttc agcaagttct cccagctcag cctggctgcc
     1921 gaggacgcca ggcagtatgc acccagcata aaagccaagt tccctgcaga cacctacgct
     1981 ctgaccgggc agatggtgac tctggagtgt ttcgcctttg ggaaccccgt tcctcaaata
     2041 aagtggagga agctggatgg ctcgcagacc tccaagtggc tctccagtga gcccctcctg
     2101 cacatccaaa atgttgactt tgaagatgaa ggcacttatg agtgcgaggc tgaaaacatc
     2161 aaagggaggg acacctacca gggccgcatc atcattcacg ctcagccgga ctggctggat
     2221 gtgatcacgg acacggaagc tgacattggg tccgacctgc gctggagctg cgtggcatcc
     2281 ggcaaacccc ggccagcagt gcggtggctg cgggatgggc aacctctggc ctcccagaac
     2341 cgcatcgaag tgagcggtgg agagctgaga ttttccaagc tagtcctgga ggactctggc
     2401 atgtatcagt gtgtggctga gaacaagcat ggcacagtat atgcaagtgc tgaattaaca
     2461 gtgcaagcct tagcaccaga ttttagacta aacccagtta aacgactgat acctgcagcc
     2521 cggagtggga aggtcatcat cccatgccaa ccgagagcag caccaaaagc cactgtgctc
     2581 tggaccaaag ggaccgaact gctcaccaac agcagcaggg tgactattac tgctgatggc
     2641 accttgatcc tccagaacat aagcaaatct gatgagggaa agtatacctg ctttgctgag
     2701 aatttcatgg gcaaagccaa cagcacgggg atcctctctg ttcgagatgc caccaaaatc
     2761 acactggcac catccagcgc tgatatcaat gtgggtgaaa acctcactct gcagtgccac
     2821 gcatcccacg acccaaccat ggacctaacc ttcacctggt cactggatga tttccccatt
     2881 gacttggaca agtcggaggg gcattaccgg cgagccagcg tgaaggaagc tgttggggac
     2941 cttgccatcg tcaatgccca gctgaagcac tcggggcggt acacatgcac agcccaaacg
     3001 gttgtggaca gcacttcgga gtcggccacg ctgactgtca gaggaccccc cgggccccca
     3061 gggggtgtgg tggtgagaga cattggtgac accactgtcc agctgagctg gagccgtggc
     3121 tttgacaacc acagccccat cgccaggtac agcattgagg ccaggacgct gctctccaac
     3181 aaatggaagc agatgcgcac caatcctgta aacatcgaag gcaacgccga gacagcccag
     3241 gtggtgaacc tcatcccttg gatggattac gagtttcggg tcttagcaag taacatcctt
     3301 ggagttgggg agccaagctt accctccagc aaaatccgta ccaaagaagc agcacccacg
     3361 gtggcaccat ctgggctcgg cggcggcggt ggggctccca atgagctcat catcaactgg
     3421 acgccaacac tgcgggacta ccagaatgga gacggcttcg gctacatcct gtcgtttcgt
     3481 aagaagggca ctcagggatg gctcactgcc cgcgtgcctc atgcagaatc cctgcattac
     3541 gtgtaccgca acgagagcat tgggccctac acccccttcg aggtgaagat caaagcatac
     3601 aacaggaaag gagagggacc ggagagcctc actgccatcg tgtactctgc agaagaagaa
     3661 ccaaaagtgg ctccattcag agttacggcc aaagccgttc tatcctcaga aatggatgtg
     3721 tcctgggagc cagtggagca gggagacatg actggtgtgc tgctgggcta tgagatccgg
     3781 tactggaaag acggcgataa agaggaggca gctgacagag tgagaacagc agggttggtc
     3841 acatcggctc acgtcacagg cctaaatccc aacacgaagt accacgtctc agtgagagcc
     3901 tacaaccgtg ctggagctgg gccccccagc ccctctacca acatcacgac cacaaagcca
     3961 ccaccaagga ggccaccagg caacatctcc tggactctga ctgggtctac cgtcaccatc
     4021 aagtgggacc cagtggtggc acaggcagat gagtctgcag ttacagggta caagatgctt
     4081 tacaggcagg attctcactc tgctcccacg ctgtacctgg cgagcaagag ccggattgac
     4141 atcccagtgc ctgaggactt cactcatgcc ttcgtacaga tccgggtgac ggggcctggg
     4201 ggggacggga ccccagcaga agtccacatc gtccgaaaca gtgggaccag tatgatggtg
     4261 gaagactccg tgacgaggcc ggtgccacac gctgccatca tcaccaccaa ctccctggcc
     4321 atggtggccc tgatccgcta cctggagctc tgatggggcc ggtgaagctg ggatgtggga
     4381 cgtgcctcct cccagggcag caggaactgc tcaatgtttg gtgctgcacc tccgtgtttg
     4441 gaccgcagca gtgttacaga gagggatttg ctagcattat ctgaagggag gaagaaagaa
     4501 acagcttttt tgttgttgtt gttcctttgg aagagtatga gattccatac aagacatgga
     4561 tctgcaacca atcgacatct tttttaagaa atgagcaaaa caaagaagaa ttctacccaa
     4621 attcagacag ccggctgccc tgcacaccgc attgagaagg tttgctgcct tacgaagcca
     4681 tgtactacaa taaccagact gctcaacttg actttttttt taacatgtac agtgtatttg
     4741 ggggaggggg aagggagacc ggggactgag gacttccacg tgatgggcac gctccacacc
     4801 gaccccaacc acagccatct ctaaggagcg atgggatgcg attcacctcc ccatcataag
     4861 agccaaattc cacagcaatg agcccaagga gacaaagcca caagagacac cgctctctgc
     4921 ttcatgctga aggagatggg tgaacacaag agttcagctt gtgaacatct ctttttttct
     4981 ttttatgaca ctggtgttaa ctcgcagcct cgtgtttgta tcagctgcca tgctgtgagg
     5041 tgcaatgttt gatgttatcc atatcccacc tggaaaactc gtgccgaatt cggatccaga
     5101 tcttattaaa gcagaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5161 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5221 tcatcaatgt atcttatcat gtctggtcga ctctagactc ttccgcttcc tcgctcactg
     5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5881 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6481 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6781 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     7201 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     7261 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7321 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7381 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7441 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     7501 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7561 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7621 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7681 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7741 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7801 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7861 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7921 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     7981 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8041 gctgcaaggc gatt
//
