LOCUS       sequence-177502-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-DHFRSTR
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..72)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             575..597
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(652..671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttagcagccg gatcagcgcg cacgcgtggt accaagcttt gtacattatt tttcgaactg
       61 cgggtggctc caagcgctgc cggcacgacg ctcgaggatt tcgaaacaat agctatgcga
      121 gttctgcgca tcagcatcgt ggaattcgct gaataccgat tcccagtcat ccggctcgta
      181 atccggaaaa tgggtgtcgc cttccacttc tgcatcgata tgcgtcagat acagtttttg
      241 cgcttttggc aagaactgtt cataaacgcg tccgccgcca atcaccatga tttctggtac
      301 gtcaccacac gccgcgatgg cttcgtcgac cgatttaacc caggtaaccc gatcatcggt
      361 cccgggttga ctgctgagga taatattttt gcggccgggc aaaggcctac cgattgattc
      421 ccaggtatgg cgccccataa tcacgggttt atttaaggtg ttgcgtttaa accaggcgag
      481 atcggcaggc aggttccatg gcatggcgtt ttccatgccg ataacgcgat ctaccgctag
      541 cgccgcaatc agactgatgg atcccatatg tatatctcct tcttaaagtt aaacaaaatt
      601 attgaattcc aattggagct ctccggagcc ggagggaaac cgttgtggtc tccctatagt
      661 gagtcgtatt aatttcgcgg gatcgatgca tggtgccaac acctacatct gtattaacga
      721 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
      781 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
      841 gcatcctctc tcgtttcatc ggtatcatta ccccatgaac agaaatcccc cttacacgga
      901 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
      961 gacattaacg cttctggaga aa
//
