LOCUS       sequence-177503-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-DHFRFLAG
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             512..534
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(589..608)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(590..608)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atcgtccttg tagtcgccgg cacgacgctc gaggatttcg aaacaatagc tatgcgagtt
       61 ctgcgcatca gcatcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc
      121 cggaaaatgg gtgtcgcctt ccacttctgc atcgatatgc gtcagataca gtttttgcgc
      181 ttttggcaag aactgttcat aaacgcgtcc gccgccaatc accatgattt ctggtacgtc
      241 accacacgcc gcgatggctt cgtcgaccga tttaacccag gtaacccgat catcggtccc
      301 gggttgactg ctgaggataa tatttttgcg gccgggcaaa ggcctaccga ttgattccca
      361 ggtatggcgc cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc
      421 ggcaggcagg ttccatggca tggcgttttc catgccgata acgcgatcta ccgctagcgc
      481 cgcaatcaga ctgatggatc ccatatgtat atctccttct taaagttaaa caaaattatt
      541 gaattccaat tggagctctc cggagccgga gggaaaccgt tgtggtctcc ctatagtgag
      601 tcgtatta
//
