LOCUS       sequence-177507-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-HISDHFR
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(542..559)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             579..601
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(644..663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(645..663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tcagcgcgca cgcgtggtac cgtacattag ccggcacgac
       61 gctcgaggat ttcgaaacaa tagctatgcg agttctgcgc atcagcatcg tggaattcgc
      121 tgaataccga ttcccagtca tccggctcgt aatccggaaa atgggtgtcg ccttccactt
      181 ctgcatcgat atgcgtcaga tacagttttt gcgcttttgg caagaactgt tcataaacgc
      241 gtccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcgtcga
      301 ccgatttaac ccaggtaacc cgatcatcgg tcccgggttg actgctgagg ataatatttt
      361 tgcggccggg caaaggccta ccgattgatt cccaggtatg gcgccccata atcacgggtt
      421 tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccat ggcatggcgt
      481 tttccatgcc gataacgcga tctaccgcta gcgccgcaat cagactgatg gatccgctgc
      541 tgtgatgatg atgatgatgg ctgctgccca tatgtatatc tccttcttaa agttaaacaa
      601 aattattgag ctctccggag ccggagggaa accgttgtgg tctccctata gtgagtcgta
      661 ttaatttcgc gggatcgatg catggtgcca acacctacat ctgtattaac gaagcgctgg
      721 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
      781 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
      841 tctcgtttca tcggtatcat taccccatga acagaaatcc cccttacacg gaggcatcag
      901 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
      961 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     1021 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgttt
//
