LOCUS       sequence-177510-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 3 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc3-CBPDHFR
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(533..610)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             624..646
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(689..708)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(690..708)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatcagcgc gcacgcgtgg tacctgtaca ttagccggca cgacgctcga
       61 ggatttcgaa acaatagcta tgcgagttct gcgcatcagc atcgtggaat tcgctgaata
      121 ccgattccca gtcatccggc tcgtaatccg gaaaatgggt gtcgccttcc acttctgcat
      181 cgatatgcgt cagatacagt ttttgcgctt ttggcaagaa ctgttcataa acgcgtccgc
      241 cgccaatcac catgatttct ggtacgtcac cacacgccgc gatggcttcg tcgaccgatt
      301 taacccaggt aacccgatca tcggtcccgg gttgactgct gaggataata tttttgcggc
      361 cgggcaaagg cctaccgatt gattcccagg tatggcgccc cataatcacg ggtttattta
      421 aggtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccatggcatg gcgttttcca
      481 tgccgataac gcgatctacc gctagcgccg caatcagact gatggatccc agaagtgccc
      541 cggaggatga gattttctta aagcggttgg ctgctgagac ggctatgaaa ttctttttcc
      601 atcgtcgctt agacatatgt atatctcctt cttaaagtta aacaaaatta ttgagctctc
      661 cggagccgga gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat
      721 cgatgcatgg tgccaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
      781 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
      841 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
      901 atcattaccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
      961 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     1021 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     1081 atgagcttta ccgcagctgc ct
//
